Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ †
In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodop...
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ftpubmed:oai:pubmedcentral.nih.gov:2813027 2023-05-15T17:33:31+02:00 Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens 2010-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813027 http://www.ncbi.nlm.nih.gov/pubmed/19948850 https://doi.org/10.1128/AEM.01525-09 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813027 http://www.ncbi.nlm.nih.gov/pubmed/19948850 http://dx.doi.org/10.1128/AEM.01525-09 Copyright © 2010, American Society for Microbiology Environmental Microbiology Text 2010 ftpubmed https://doi.org/10.1128/AEM.01525-09 2013-09-02T20:58:56Z In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. Text North Atlantic PubMed Central (PMC) Applied and Environmental Microbiology 76 3 776 785 |
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Environmental Microbiology |
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Environmental Microbiology Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
topic_facet |
Environmental Microbiology |
description |
In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. |
format |
Text |
author |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens |
author_facet |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens |
author_sort |
Winkelmann, Nadine |
title |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
title_short |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
title_full |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
title_fullStr |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
title_full_unstemmed |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis ▿ † |
title_sort |
determination of the diversity of rhodopirellula isolates from european seas by multilocus sequence analysis ▿ † |
publisher |
American Society for Microbiology (ASM) |
publishDate |
2010 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813027 http://www.ncbi.nlm.nih.gov/pubmed/19948850 https://doi.org/10.1128/AEM.01525-09 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813027 http://www.ncbi.nlm.nih.gov/pubmed/19948850 http://dx.doi.org/10.1128/AEM.01525-09 |
op_rights |
Copyright © 2010, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.01525-09 |
container_title |
Applied and Environmental Microbiology |
container_volume |
76 |
container_issue |
3 |
container_start_page |
776 |
op_container_end_page |
785 |
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1766132055779835904 |