Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae
The tissue-specific gene expression of the vitamin K-dependent proteins bone γ-carboxyglutamate-protein (BGP) and matrix γ-carboxyglutamate-protein (MGP) in Atlantic salmon (Salmo salar L.) was investigated. In previous studies, BGP, the most abundant non-collagenous protein of bone, was almost excl...
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ftpubmed:oai:pubmedcentral.nih.gov:2796789 2023-05-15T15:28:48+02:00 Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae Krossøy, Christel Ørnsrud, Robin Wargelius, Anna 2009-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796789 http://www.ncbi.nlm.nih.gov/pubmed/19811564 https://doi.org/10.1111/j.1469-7580.2009.01153.x en eng Blackwell Science Inc http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796789 http://www.ncbi.nlm.nih.gov/pubmed/19811564 http://dx.doi.org/10.1111/j.1469-7580.2009.01153.x Journal compilation © 2009 Anatomical Society of Great Britain and Ireland Original Articles Text 2009 ftpubmed https://doi.org/10.1111/j.1469-7580.2009.01153.x 2013-09-02T20:01:03Z The tissue-specific gene expression of the vitamin K-dependent proteins bone γ-carboxyglutamate-protein (BGP) and matrix γ-carboxyglutamate-protein (MGP) in Atlantic salmon (Salmo salar L.) was investigated. In previous studies, BGP, the most abundant non-collagenous protein of bone, was almost exclusively associated with bone, whereas the non-structural protein MGP has a more widespread tissue distribution. In-situ hybridization of juvenile Atlantic salmon (∼40 g, fresh water) vertebrae demonstrated expression of bgp and mgp mRNA in osteoblasts lining the trabecular bone, whereas no staining was observed in the compact bone. By separating the trabecular and compact bone of both juvenile (∼40 g, fresh water) and adult (∼1000 g, sea water) Atlantic salmon, we observed that the two vertebral bone compartments displayed different levels of bgp, whereas no such differences were seen for mgp. Measurements of the mineral content and Ca/P molar ratio in adult salmon revealed no significant differences between trabecular and compact bone. In conclusion, the osteoblasts covering the salmon vertebrae have unique gene expression patterns and levels of bgp and mgp. Further, the study confirms the presence of mRNA from the vitamin K-dependent proteins BGP and MGP in the vertebrae, fin and gills of Atlantic salmon. Text Atlantic salmon Salmo salar PubMed Central (PMC) Journal of Anatomy 215 6 663 672 |
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Original Articles Krossøy, Christel Ørnsrud, Robin Wargelius, Anna Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
topic_facet |
Original Articles |
description |
The tissue-specific gene expression of the vitamin K-dependent proteins bone γ-carboxyglutamate-protein (BGP) and matrix γ-carboxyglutamate-protein (MGP) in Atlantic salmon (Salmo salar L.) was investigated. In previous studies, BGP, the most abundant non-collagenous protein of bone, was almost exclusively associated with bone, whereas the non-structural protein MGP has a more widespread tissue distribution. In-situ hybridization of juvenile Atlantic salmon (∼40 g, fresh water) vertebrae demonstrated expression of bgp and mgp mRNA in osteoblasts lining the trabecular bone, whereas no staining was observed in the compact bone. By separating the trabecular and compact bone of both juvenile (∼40 g, fresh water) and adult (∼1000 g, sea water) Atlantic salmon, we observed that the two vertebral bone compartments displayed different levels of bgp, whereas no such differences were seen for mgp. Measurements of the mineral content and Ca/P molar ratio in adult salmon revealed no significant differences between trabecular and compact bone. In conclusion, the osteoblasts covering the salmon vertebrae have unique gene expression patterns and levels of bgp and mgp. Further, the study confirms the presence of mRNA from the vitamin K-dependent proteins BGP and MGP in the vertebrae, fin and gills of Atlantic salmon. |
format |
Text |
author |
Krossøy, Christel Ørnsrud, Robin Wargelius, Anna |
author_facet |
Krossøy, Christel Ørnsrud, Robin Wargelius, Anna |
author_sort |
Krossøy, Christel |
title |
Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
title_short |
Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
title_full |
Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
title_fullStr |
Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
title_full_unstemmed |
Differential gene expression of bgp and mgp in trabecular and compact bone of Atlantic salmon (Salmo salar L.) vertebrae |
title_sort |
differential gene expression of bgp and mgp in trabecular and compact bone of atlantic salmon (salmo salar l.) vertebrae |
publisher |
Blackwell Science Inc |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796789 http://www.ncbi.nlm.nih.gov/pubmed/19811564 https://doi.org/10.1111/j.1469-7580.2009.01153.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796789 http://www.ncbi.nlm.nih.gov/pubmed/19811564 http://dx.doi.org/10.1111/j.1469-7580.2009.01153.x |
op_rights |
Journal compilation © 2009 Anatomical Society of Great Britain and Ireland |
op_doi |
https://doi.org/10.1111/j.1469-7580.2009.01153.x |
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Journal of Anatomy |
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215 |
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6 |
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663 |
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672 |
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1766359151627206656 |