Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases
A reconstituted human tissue model was used to mimic Candida albicans and Candida parapsilosis infection in order to investigate the protective effects of acetylsalicylic acid (aspirin, ASA). We found that therapeutic concentrations of ASA reduced tissue damage in the in vitro infection model. We fu...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787780 http://www.ncbi.nlm.nih.gov/pubmed/19703582 https://doi.org/10.1016/j.micinf.2009.08.007 |
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ftpubmed:oai:pubmedcentral.nih.gov:2787780 2023-05-15T13:38:10+02:00 Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases Trofa, David Agovino, Mariangela Stehr, Frank Schäfer, Wilhelm Rykunov, Dmitry Fiser, András Hamari, Zsuzsanna Nosanchuk, Joshua D. Gácser, Attila 2009-08-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787780 http://www.ncbi.nlm.nih.gov/pubmed/19703582 https://doi.org/10.1016/j.micinf.2009.08.007 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787780 http://www.ncbi.nlm.nih.gov/pubmed/19703582 http://dx.doi.org/10.1016/j.micinf.2009.08.007 Article Text 2009 ftpubmed https://doi.org/10.1016/j.micinf.2009.08.007 2013-09-02T19:28:05Z A reconstituted human tissue model was used to mimic Candida albicans and Candida parapsilosis infection in order to investigate the protective effects of acetylsalicylic acid (aspirin, ASA). We found that therapeutic concentrations of ASA reduced tissue damage in the in vitro infection model. We further evaluated the lipase inhibitory effects of ASA by investigating the growth of C. albicans, C. parapsilosis and C. parapsilosis lipase negative (Δcplip1-2/Δcplip1-2) mutants in a lipid rich minimal medium supplemented with olive oil and found that a therapeutic concentration of ASA inhibited the growth of wild type fungi. The lipase inhibitors quinine and ebelactone B were also shown to reduce growth and protect against tissue damage from Candida species, respectively. A lipolytic activity assay also showed that therapeutic concentrations of ASA inhibited C. antarctica and C. cylindracea purified lipases obtained through a commercial kit. The relationship between ASA and lipase was characterized through a computed structural model of the Lipase-2 protein from C. parapsilosis in complex with ASA. The results suggest that development of inhibitors of fungal lipases could result in broad-spectrum therapeutics, especially since fungal lipases are not homologous to their human analogues. Text Antarc* Antarctica PubMed Central (PMC) Microbes and Infection 11 14-15 1131 1139 |
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Article Trofa, David Agovino, Mariangela Stehr, Frank Schäfer, Wilhelm Rykunov, Dmitry Fiser, András Hamari, Zsuzsanna Nosanchuk, Joshua D. Gácser, Attila Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
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Article |
description |
A reconstituted human tissue model was used to mimic Candida albicans and Candida parapsilosis infection in order to investigate the protective effects of acetylsalicylic acid (aspirin, ASA). We found that therapeutic concentrations of ASA reduced tissue damage in the in vitro infection model. We further evaluated the lipase inhibitory effects of ASA by investigating the growth of C. albicans, C. parapsilosis and C. parapsilosis lipase negative (Δcplip1-2/Δcplip1-2) mutants in a lipid rich minimal medium supplemented with olive oil and found that a therapeutic concentration of ASA inhibited the growth of wild type fungi. The lipase inhibitors quinine and ebelactone B were also shown to reduce growth and protect against tissue damage from Candida species, respectively. A lipolytic activity assay also showed that therapeutic concentrations of ASA inhibited C. antarctica and C. cylindracea purified lipases obtained through a commercial kit. The relationship between ASA and lipase was characterized through a computed structural model of the Lipase-2 protein from C. parapsilosis in complex with ASA. The results suggest that development of inhibitors of fungal lipases could result in broad-spectrum therapeutics, especially since fungal lipases are not homologous to their human analogues. |
format |
Text |
author |
Trofa, David Agovino, Mariangela Stehr, Frank Schäfer, Wilhelm Rykunov, Dmitry Fiser, András Hamari, Zsuzsanna Nosanchuk, Joshua D. Gácser, Attila |
author_facet |
Trofa, David Agovino, Mariangela Stehr, Frank Schäfer, Wilhelm Rykunov, Dmitry Fiser, András Hamari, Zsuzsanna Nosanchuk, Joshua D. Gácser, Attila |
author_sort |
Trofa, David |
title |
Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
title_short |
Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
title_full |
Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
title_fullStr |
Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
title_full_unstemmed |
Acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with Candida species by inhibiting extracellular fungal lipases |
title_sort |
acetylsalicylic acid (aspirin) reduces damage to reconstituted human tissues infected with candida species by inhibiting extracellular fungal lipases |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787780 http://www.ncbi.nlm.nih.gov/pubmed/19703582 https://doi.org/10.1016/j.micinf.2009.08.007 |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787780 http://www.ncbi.nlm.nih.gov/pubmed/19703582 http://dx.doi.org/10.1016/j.micinf.2009.08.007 |
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https://doi.org/10.1016/j.micinf.2009.08.007 |
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Microbes and Infection |
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11 |
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14-15 |
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1131 |
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1139 |
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1766102065538400256 |