Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)
Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scopht...
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ftpubmed:oai:pubmedcentral.nih.gov:2787431 2023-05-15T18:15:43+02:00 Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago 2009-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787431 http://www.ncbi.nlm.nih.gov/pubmed/19786621 https://doi.org/10.1534/genetics.109.107979 en eng Genetics Society of America http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787431 http://www.ncbi.nlm.nih.gov/pubmed/19786621 http://dx.doi.org/10.1534/genetics.109.107979 Copyright © 2009 by the Genetics Society of America Investigations Text 2009 ftpubmed https://doi.org/10.1534/genetics.109.107979 2013-09-02T19:26:46Z Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species. Text Scophthalmus maximus Turbot PubMed Central (PMC) Genetics 183 4 1443 1452 |
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Investigations Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
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Investigations |
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Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species. |
format |
Text |
author |
Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago |
author_facet |
Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago |
author_sort |
Martínez, Paulino |
title |
Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
title_short |
Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
title_full |
Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
title_fullStr |
Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
title_full_unstemmed |
Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus) |
title_sort |
identification of the major sex-determining region of turbot (scophthalmus maximus) |
publisher |
Genetics Society of America |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787431 http://www.ncbi.nlm.nih.gov/pubmed/19786621 https://doi.org/10.1534/genetics.109.107979 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787431 http://www.ncbi.nlm.nih.gov/pubmed/19786621 http://dx.doi.org/10.1534/genetics.109.107979 |
op_rights |
Copyright © 2009 by the Genetics Society of America |
op_doi |
https://doi.org/10.1534/genetics.109.107979 |
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Genetics |
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183 |
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4 |
container_start_page |
1443 |
op_container_end_page |
1452 |
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1766188923334164480 |