Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel
During the pre-hibernation season, arctic ground squirrels (AGS) can tolerate 8 minutes of asphyxial cardiac arrest (CA) without detectable brain pathology. Better understanding of the mechanisms regulating innate ischemia tolerance in AGS has the potential to facilitate the development of novel, pr...
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ftpubmed:oai:pubmedcentral.nih.gov:2774829 2023-05-15T14:31:29+02:00 Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel Dave, Kunjan R. DeFazio, R. Anthony Raval, Ami P. Dashkin, Oleksandr Saul, Isabel Iceman, Kimberly E. Perez-Pinzon, Miguel A. Drew, Kelly L. 2009-05-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774829 http://www.ncbi.nlm.nih.gov/pubmed/19493168 https://doi.org/10.1111/j.1471-4159.2009.06196.x en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774829 http://www.ncbi.nlm.nih.gov/pubmed/19493168 http://dx.doi.org/10.1111/j.1471-4159.2009.06196.x Article Text 2009 ftpubmed https://doi.org/10.1111/j.1471-4159.2009.06196.x 2013-09-02T18:40:11Z During the pre-hibernation season, arctic ground squirrels (AGS) can tolerate 8 minutes of asphyxial cardiac arrest (CA) without detectable brain pathology. Better understanding of the mechanisms regulating innate ischemia tolerance in AGS has the potential to facilitate the development of novel, prophylactic agents to induce ischemic tolerance in patients at risk of stroke or cardiac arrest. We hypothesized that neuroprotection in AGS involves robust maintenance of ion homeostasis similar to anoxia-tolerant turtles. Ion homeostasis was assessed by monitoring ischemic depolarization (ID) in cerebral cortex during CA in vivo and during oxygen glucose deprivation in vitro in acutely prepared hippocampal slices. In both models, the onset of ID was significantly delayed in AGS compared to rats. The epsilon protein kinase C (εPKC) is a key mediator of neuroprotection and inhibits both Na+/K+-ATPase and voltage-gated sodium channels, primary mediators of the collapse of ion homeostasis during ischemia. The selective peptide inhibitor of εPKC (εV1–2) shortened the time to ID in brain slices from AGS but not in rats despite evidence that εV1–2 decreased activation of εPKC in brain slices from both rats and AGS. These results support the hypothesis that εPKC activation delays the collapse of ion homeostasis during ischemia in AGS. Text Arctic ground squirrel Arctic PubMed Central (PMC) Arctic Journal of Neurochemistry 110 4 1170 1179 |
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Article Dave, Kunjan R. DeFazio, R. Anthony Raval, Ami P. Dashkin, Oleksandr Saul, Isabel Iceman, Kimberly E. Perez-Pinzon, Miguel A. Drew, Kelly L. Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
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Article |
description |
During the pre-hibernation season, arctic ground squirrels (AGS) can tolerate 8 minutes of asphyxial cardiac arrest (CA) without detectable brain pathology. Better understanding of the mechanisms regulating innate ischemia tolerance in AGS has the potential to facilitate the development of novel, prophylactic agents to induce ischemic tolerance in patients at risk of stroke or cardiac arrest. We hypothesized that neuroprotection in AGS involves robust maintenance of ion homeostasis similar to anoxia-tolerant turtles. Ion homeostasis was assessed by monitoring ischemic depolarization (ID) in cerebral cortex during CA in vivo and during oxygen glucose deprivation in vitro in acutely prepared hippocampal slices. In both models, the onset of ID was significantly delayed in AGS compared to rats. The epsilon protein kinase C (εPKC) is a key mediator of neuroprotection and inhibits both Na+/K+-ATPase and voltage-gated sodium channels, primary mediators of the collapse of ion homeostasis during ischemia. The selective peptide inhibitor of εPKC (εV1–2) shortened the time to ID in brain slices from AGS but not in rats despite evidence that εV1–2 decreased activation of εPKC in brain slices from both rats and AGS. These results support the hypothesis that εPKC activation delays the collapse of ion homeostasis during ischemia in AGS. |
format |
Text |
author |
Dave, Kunjan R. DeFazio, R. Anthony Raval, Ami P. Dashkin, Oleksandr Saul, Isabel Iceman, Kimberly E. Perez-Pinzon, Miguel A. Drew, Kelly L. |
author_facet |
Dave, Kunjan R. DeFazio, R. Anthony Raval, Ami P. Dashkin, Oleksandr Saul, Isabel Iceman, Kimberly E. Perez-Pinzon, Miguel A. Drew, Kelly L. |
author_sort |
Dave, Kunjan R. |
title |
Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
title_short |
Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
title_full |
Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
title_fullStr |
Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
title_full_unstemmed |
Protein kinase C epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
title_sort |
protein kinase c epsilon activation delays neuronal depolarization during cardiac arrest in the euthermic arctic ground squirrel |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774829 http://www.ncbi.nlm.nih.gov/pubmed/19493168 https://doi.org/10.1111/j.1471-4159.2009.06196.x |
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Arctic |
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Arctic |
genre |
Arctic ground squirrel Arctic |
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Arctic ground squirrel Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774829 http://www.ncbi.nlm.nih.gov/pubmed/19493168 http://dx.doi.org/10.1111/j.1471-4159.2009.06196.x |
op_doi |
https://doi.org/10.1111/j.1471-4159.2009.06196.x |
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Journal of Neurochemistry |
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110 |
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4 |
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1170 |
op_container_end_page |
1179 |
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1766305106732515328 |