Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ †
Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the developmen...
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ftpubmed:oai:pubmedcentral.nih.gov:2747875 2023-05-15T15:46:20+02:00 Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † Lu, Jingrang Santo Domingo, Jorge W. Hill, Stephen Edge, Thomas A. 2009-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747875 http://www.ncbi.nlm.nih.gov/pubmed/19633110 https://doi.org/10.1128/AEM.00462-09 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747875 http://www.ncbi.nlm.nih.gov/pubmed/19633110 http://dx.doi.org/10.1128/AEM.00462-09 Copyright © 2009, American Society for Microbiology Environmental Microbiology Text 2009 ftpubmed https://doi.org/10.1128/AEM.00462-09 2013-09-02T17:01:34Z Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the development of host-specific source-tracking methods. To address this issue, 16S rRNA gene clone libraries for Canada goose fecal samples from Ontario, Canada, and Ohio were analyzed. Analyses of fecal clones from Ontario (447) and Ohio (302) showed that goose fecal communities are dominated by the classes “Clostridia” (represented by 33.7% of clones) and “Bacilli” (38.1% of clones) and the phylum “Bacteroidetes” (10.1% of clones). Sequences not previously found in other avian fecal communities were used to develop host-specific assays. Fecal DNA extracts from sewage plants (10 samples) and different species of birds (11 samples) and mammals (18 samples) were used to test for host specificity. Of all the assays tested, one assay showed specificity for Canada goose fecal DNA. The PCR assay was positive for Canada goose fecal DNA extracts collected from three locations in North America (Ohio, Oregon, and Ontario, Canada). Additionally, of 48 DNA extracts from Lake Ontario waters presumed to be impacted by waterfowl feces, 19 tested positive by the assay, although 10 were positive only after a nested PCR approach was used. Due to the level of host specificity and the presence of signals in environmental waters, the assay is proposed as a part of the toolbox to detect Canada goose contamination in waterfowl-contaminated waters. Text Branta canadensis Canada Goose PubMed Central (PMC) Canada Applied and Environmental Microbiology 75 18 5919 5926 |
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Environmental Microbiology |
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Environmental Microbiology Lu, Jingrang Santo Domingo, Jorge W. Hill, Stephen Edge, Thomas A. Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
topic_facet |
Environmental Microbiology |
description |
Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the development of host-specific source-tracking methods. To address this issue, 16S rRNA gene clone libraries for Canada goose fecal samples from Ontario, Canada, and Ohio were analyzed. Analyses of fecal clones from Ontario (447) and Ohio (302) showed that goose fecal communities are dominated by the classes “Clostridia” (represented by 33.7% of clones) and “Bacilli” (38.1% of clones) and the phylum “Bacteroidetes” (10.1% of clones). Sequences not previously found in other avian fecal communities were used to develop host-specific assays. Fecal DNA extracts from sewage plants (10 samples) and different species of birds (11 samples) and mammals (18 samples) were used to test for host specificity. Of all the assays tested, one assay showed specificity for Canada goose fecal DNA. The PCR assay was positive for Canada goose fecal DNA extracts collected from three locations in North America (Ohio, Oregon, and Ontario, Canada). Additionally, of 48 DNA extracts from Lake Ontario waters presumed to be impacted by waterfowl feces, 19 tested positive by the assay, although 10 were positive only after a nested PCR approach was used. Due to the level of host specificity and the presence of signals in environmental waters, the assay is proposed as a part of the toolbox to detect Canada goose contamination in waterfowl-contaminated waters. |
format |
Text |
author |
Lu, Jingrang Santo Domingo, Jorge W. Hill, Stephen Edge, Thomas A. |
author_facet |
Lu, Jingrang Santo Domingo, Jorge W. Hill, Stephen Edge, Thomas A. |
author_sort |
Lu, Jingrang |
title |
Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
title_short |
Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
title_full |
Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
title_fullStr |
Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
title_full_unstemmed |
Microbial Diversity and Host-Specific Sequences of Canada Goose Feces▿ † |
title_sort |
microbial diversity and host-specific sequences of canada goose feces▿ † |
publisher |
American Society for Microbiology (ASM) |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747875 http://www.ncbi.nlm.nih.gov/pubmed/19633110 https://doi.org/10.1128/AEM.00462-09 |
geographic |
Canada |
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Canada |
genre |
Branta canadensis Canada Goose |
genre_facet |
Branta canadensis Canada Goose |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747875 http://www.ncbi.nlm.nih.gov/pubmed/19633110 http://dx.doi.org/10.1128/AEM.00462-09 |
op_rights |
Copyright © 2009, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.00462-09 |
container_title |
Applied and Environmental Microbiology |
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75 |
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18 |
container_start_page |
5919 |
op_container_end_page |
5926 |
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1766381032136769536 |