Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence a...
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ftpubmed:oai:pubmedcentral.nih.gov:2739905 2023-05-15T18:15:40+02:00 Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus Jia, Airong Zhang, Xiao-Hua 2009 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739905 http://www.ncbi.nlm.nih.gov/pubmed/19750236 https://doi.org/10.1155/2009/767893 en eng Hindawi Publishing Corporation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739905 http://www.ncbi.nlm.nih.gov/pubmed/19750236 http://dx.doi.org/10.1155/2009/767893 Copyright © 2009 A. Jia and X.-H. Zhang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2009 ftpubmed https://doi.org/10.1155/2009/767893 2013-09-02T16:32:23Z Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. Text Scophthalmus maximus Turbot PubMed Central (PMC) Journal of Biomedicine and Biotechnology 2009 1 8 |
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Research Article Jia, Airong Zhang, Xiao-Hua Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
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Research Article |
description |
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. |
format |
Text |
author |
Jia, Airong Zhang, Xiao-Hua |
author_facet |
Jia, Airong Zhang, Xiao-Hua |
author_sort |
Jia, Airong |
title |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_short |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_fullStr |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full_unstemmed |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_sort |
molecular cloning, characterization, and expression analysis of the cxcr4 gene from turbot: scophthalmus maximus |
publisher |
Hindawi Publishing Corporation |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739905 http://www.ncbi.nlm.nih.gov/pubmed/19750236 https://doi.org/10.1155/2009/767893 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2739905 http://www.ncbi.nlm.nih.gov/pubmed/19750236 http://dx.doi.org/10.1155/2009/767893 |
op_rights |
Copyright © 2009 A. Jia and X.-H. Zhang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2009/767893 |
container_title |
Journal of Biomedicine and Biotechnology |
container_volume |
2009 |
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1 |
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8 |
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1766188849885609984 |