Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae
A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated gen...
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ftpubmed:oai:pubmedcentral.nih.gov:2620446 2023-05-15T14:01:57+02:00 Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. 2000-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620446 http://www.ncbi.nlm.nih.gov/pubmed/19270960 en eng Society of Nematologists http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620446 http://www.ncbi.nlm.nih.gov/pubmed/19270960 © The Society of Nematologists 2000 Molecular Karyotypic and Behavior Evolution Text 2000 ftpubmed 2013-09-02T09:42:13Z A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated genetic diversity in this nematode using nuclear and mitochondrial gene sequences that encode ribosomal RNA. In 169 nematodes surveyed, only one variable site was found in each of two different expansion segments of nuclear rRNA. While most nematodes have only one sequence type, some nematodes were found to contain a mixture of both sequences. No fixed differences in nuclear sequences were observed between populations. This pattern of nuclear variation is most consistent with a single species of nematode defined morphologically as S. lindsayae. For mitochondrial DNA sequences, we found 10 variable positions defining 12 haplotypes among 188 nematodes surveyed. While all observed haplotypes are closely related, significant differences in haplotype frequencies were observed between geographically defined populations. The nuclear and mitochondrial variation suggests populations of S. lindsayae represent a single polymorphic species with some restriction of gene flow between geographic populations. Text Antarc* Antarctic Antarctica McMurdo Dry Valleys PubMed Central (PMC) Antarctic McMurdo Dry Valleys The Antarctic |
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English |
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Molecular Karyotypic and Behavior Evolution |
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Molecular Karyotypic and Behavior Evolution Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
topic_facet |
Molecular Karyotypic and Behavior Evolution |
description |
A nematode, Scottnema lindsayae, is the dominant metazoan found in soils of the McMurdo Dry Valleys, Antarctica. The distribution of S. lindsayae is patchy within and between these dry valleys; nevertheless, it is unclear to what extent these populations are genetically isolated. We investigated genetic diversity in this nematode using nuclear and mitochondrial gene sequences that encode ribosomal RNA. In 169 nematodes surveyed, only one variable site was found in each of two different expansion segments of nuclear rRNA. While most nematodes have only one sequence type, some nematodes were found to contain a mixture of both sequences. No fixed differences in nuclear sequences were observed between populations. This pattern of nuclear variation is most consistent with a single species of nematode defined morphologically as S. lindsayae. For mitochondrial DNA sequences, we found 10 variable positions defining 12 haplotypes among 188 nematodes surveyed. While all observed haplotypes are closely related, significant differences in haplotype frequencies were observed between geographically defined populations. The nuclear and mitochondrial variation suggests populations of S. lindsayae represent a single polymorphic species with some restriction of gene flow between geographic populations. |
format |
Text |
author |
Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. |
author_facet |
Courtright, E. M. Wall, D. H. Virginia, R. A. Frisse, L. M. Vida, J. T. Thomas, W. K. |
author_sort |
Courtright, E. M. |
title |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_short |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_full |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_fullStr |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_full_unstemmed |
Nuclear and Mitochondrial DNA Sequence Diversity in the Antarctic Nematode Scottnema lindsayae |
title_sort |
nuclear and mitochondrial dna sequence diversity in the antarctic nematode scottnema lindsayae |
publisher |
Society of Nematologists |
publishDate |
2000 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620446 http://www.ncbi.nlm.nih.gov/pubmed/19270960 |
geographic |
Antarctic McMurdo Dry Valleys The Antarctic |
geographic_facet |
Antarctic McMurdo Dry Valleys The Antarctic |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620446 http://www.ncbi.nlm.nih.gov/pubmed/19270960 |
op_rights |
© The Society of Nematologists 2000 |
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1766272018959826944 |