Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †

A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fibers at e...

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Published in:Journal of Bacteriology
Main Authors: Vestergaard, Gisle, Shah, Shiraz A., Bize, Ariane, Reitberger, Werner, Reuter, Monika, Phan, Hien, Briegel, Ariane, Rachel, Reinhard, Garrett, Roger A., Prangishvili, David
Format: Text
Language:English
Published: American Society for Microbiology (ASM) 2008
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566220
http://www.ncbi.nlm.nih.gov/pubmed/18723627
https://doi.org/10.1128/JB.00795-08
id ftpubmed:oai:pubmedcentral.nih.gov:2566220
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spelling ftpubmed:oai:pubmedcentral.nih.gov:2566220 2023-05-15T16:51:53+02:00 Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ † Vestergaard, Gisle Shah, Shiraz A. Bize, Ariane Reitberger, Werner Reuter, Monika Phan, Hien Briegel, Ariane Rachel, Reinhard Garrett, Roger A. Prangishvili, David 2008-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566220 http://www.ncbi.nlm.nih.gov/pubmed/18723627 https://doi.org/10.1128/JB.00795-08 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566220 http://www.ncbi.nlm.nih.gov/pubmed/18723627 http://dx.doi.org/10.1128/JB.00795-08 Copyright © 2008, American Society for Microbiology Genetics and Molecular Biology Text 2008 ftpubmed https://doi.org/10.1128/JB.00795-08 2013-09-02T06:54:38Z A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fibers at each terminus. The linear double-stranded DNA genome contains 28,096 bp and an inverted terminal repeat of 1,030 bp. The SRV shows morphological and genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy. The single major rudiviral structural protein is shown to generate long tubular structures in vitro of similar dimensions to those of the virion, and we estimate that the virion constitutes a single, superhelical, double-stranded DNA embedded into such a protein structure. Three additional minor conserved structural proteins are also identified. Ubiquitous rudiviral proteins with assigned functions include glycosyl transferases and a S-adenosylmethionine-dependent methyltransferase, as well as a Holliday junction resolvase, a transcriptionally coupled helicase and nuclease implicated in DNA replication. Analysis of matches between known crenarchaeal chromosomal CRISPR spacer sequences, implicated in a viral defense system, and rudiviral genomes revealed that about 10% of the 3,042 unique acidothermophile spacers yield significant matches to rudiviral genomes, with a bias to highly conserved protein genes, consistent with the widespread presence of rudiviruses in hot acidophilic environments. We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system. Text Iceland PubMed Central (PMC) Journal of Bacteriology 190 20 6837 6845
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Genetics and Molecular Biology
spellingShingle Genetics and Molecular Biology
Vestergaard, Gisle
Shah, Shiraz A.
Bize, Ariane
Reitberger, Werner
Reuter, Monika
Phan, Hien
Briegel, Ariane
Rachel, Reinhard
Garrett, Roger A.
Prangishvili, David
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
topic_facet Genetics and Molecular Biology
description A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fibers at each terminus. The linear double-stranded DNA genome contains 28,096 bp and an inverted terminal repeat of 1,030 bp. The SRV shows morphological and genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy. The single major rudiviral structural protein is shown to generate long tubular structures in vitro of similar dimensions to those of the virion, and we estimate that the virion constitutes a single, superhelical, double-stranded DNA embedded into such a protein structure. Three additional minor conserved structural proteins are also identified. Ubiquitous rudiviral proteins with assigned functions include glycosyl transferases and a S-adenosylmethionine-dependent methyltransferase, as well as a Holliday junction resolvase, a transcriptionally coupled helicase and nuclease implicated in DNA replication. Analysis of matches between known crenarchaeal chromosomal CRISPR spacer sequences, implicated in a viral defense system, and rudiviral genomes revealed that about 10% of the 3,042 unique acidothermophile spacers yield significant matches to rudiviral genomes, with a bias to highly conserved protein genes, consistent with the widespread presence of rudiviruses in hot acidophilic environments. We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system.
format Text
author Vestergaard, Gisle
Shah, Shiraz A.
Bize, Ariane
Reitberger, Werner
Reuter, Monika
Phan, Hien
Briegel, Ariane
Rachel, Reinhard
Garrett, Roger A.
Prangishvili, David
author_facet Vestergaard, Gisle
Shah, Shiraz A.
Bize, Ariane
Reitberger, Werner
Reuter, Monika
Phan, Hien
Briegel, Ariane
Rachel, Reinhard
Garrett, Roger A.
Prangishvili, David
author_sort Vestergaard, Gisle
title Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
title_short Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
title_full Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
title_fullStr Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
title_full_unstemmed Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System▿ †
title_sort stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the crispr antiviral system▿ †
publisher American Society for Microbiology (ASM)
publishDate 2008
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566220
http://www.ncbi.nlm.nih.gov/pubmed/18723627
https://doi.org/10.1128/JB.00795-08
genre Iceland
genre_facet Iceland
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566220
http://www.ncbi.nlm.nih.gov/pubmed/18723627
http://dx.doi.org/10.1128/JB.00795-08
op_rights Copyright © 2008, American Society for Microbiology
op_doi https://doi.org/10.1128/JB.00795-08
container_title Journal of Bacteriology
container_volume 190
container_issue 20
container_start_page 6837
op_container_end_page 6845
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