Extraordinary intraspecific diversity in oyster sperm bindin
In free-spawning invertebrates sperm–egg incompatibility is a barrier to mating between species, and divergence of gamete recognition proteins (GRPs) can result in reproductive isolation. Of interest are processes that create reproductive protein diversity within species, because intraspecific varia...
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ftpubmed:oai:pubmedcentral.nih.gov:2538870 2023-05-15T15:58:43+02:00 Extraordinary intraspecific diversity in oyster sperm bindin Moy, G. W. Springer, S. A. Adams, S. L. Swanson, W. J. Vacquier, V. D. 2008-02-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2538870 http://www.ncbi.nlm.nih.gov/pubmed/18268333 https://doi.org/10.1073/pnas.0711862105 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2538870 http://www.ncbi.nlm.nih.gov/pubmed/18268333 http://dx.doi.org/10.1073/pnas.0711862105 © 2008 by The National Academy of Sciences of the USA Biological Sciences Text 2008 ftpubmed https://doi.org/10.1073/pnas.0711862105 2013-09-02T05:31:28Z In free-spawning invertebrates sperm–egg incompatibility is a barrier to mating between species, and divergence of gamete recognition proteins (GRPs) can result in reproductive isolation. Of interest are processes that create reproductive protein diversity within species, because intraspecific variants are potentially involved in mate choice and early speciation. Sperm acrosomes of the Pacific oyster Crassostrea gigas contain the protein bindin that bonds sperm to egg during fertilization. Oyster bindin is a single-copy gene encoding a diversity of protein variants. Oyster bindins have a conserved N-terminal region followed by one to five tandem fucose-binding lectin (F-lectin) domains. These repeats have diversified by positive selection at eight sites clustered on the F-lectin's fucose binding face. Additional bindin variants result from recombination in an intron in each F-lectin repeat. Males also express alternatively spliced bindin cDNAs with one to five repeats, but typically translate only one or two isoforms into protein. Thus, positive selection, alternative splicing, and recombination can create thousands of bindin variants within C. gigas. Models of sexual conflict predict high male diversity when females are diverse and sexual conflict is strong. The amount of intraspecific polymorphism in male GRPs may be a consequence of the relative efficiency of local (molecular recognition) and global (electrical, cortical, and physical) polyspermy blocks that operate during fertilization. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Proceedings of the National Academy of Sciences 105 6 1993 1998 |
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Biological Sciences |
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Biological Sciences Moy, G. W. Springer, S. A. Adams, S. L. Swanson, W. J. Vacquier, V. D. Extraordinary intraspecific diversity in oyster sperm bindin |
topic_facet |
Biological Sciences |
description |
In free-spawning invertebrates sperm–egg incompatibility is a barrier to mating between species, and divergence of gamete recognition proteins (GRPs) can result in reproductive isolation. Of interest are processes that create reproductive protein diversity within species, because intraspecific variants are potentially involved in mate choice and early speciation. Sperm acrosomes of the Pacific oyster Crassostrea gigas contain the protein bindin that bonds sperm to egg during fertilization. Oyster bindin is a single-copy gene encoding a diversity of protein variants. Oyster bindins have a conserved N-terminal region followed by one to five tandem fucose-binding lectin (F-lectin) domains. These repeats have diversified by positive selection at eight sites clustered on the F-lectin's fucose binding face. Additional bindin variants result from recombination in an intron in each F-lectin repeat. Males also express alternatively spliced bindin cDNAs with one to five repeats, but typically translate only one or two isoforms into protein. Thus, positive selection, alternative splicing, and recombination can create thousands of bindin variants within C. gigas. Models of sexual conflict predict high male diversity when females are diverse and sexual conflict is strong. The amount of intraspecific polymorphism in male GRPs may be a consequence of the relative efficiency of local (molecular recognition) and global (electrical, cortical, and physical) polyspermy blocks that operate during fertilization. |
format |
Text |
author |
Moy, G. W. Springer, S. A. Adams, S. L. Swanson, W. J. Vacquier, V. D. |
author_facet |
Moy, G. W. Springer, S. A. Adams, S. L. Swanson, W. J. Vacquier, V. D. |
author_sort |
Moy, G. W. |
title |
Extraordinary intraspecific diversity in oyster sperm bindin |
title_short |
Extraordinary intraspecific diversity in oyster sperm bindin |
title_full |
Extraordinary intraspecific diversity in oyster sperm bindin |
title_fullStr |
Extraordinary intraspecific diversity in oyster sperm bindin |
title_full_unstemmed |
Extraordinary intraspecific diversity in oyster sperm bindin |
title_sort |
extraordinary intraspecific diversity in oyster sperm bindin |
publisher |
National Academy of Sciences |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2538870 http://www.ncbi.nlm.nih.gov/pubmed/18268333 https://doi.org/10.1073/pnas.0711862105 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2538870 http://www.ncbi.nlm.nih.gov/pubmed/18268333 http://dx.doi.org/10.1073/pnas.0711862105 |
op_rights |
© 2008 by The National Academy of Sciences of the USA |
op_doi |
https://doi.org/10.1073/pnas.0711862105 |
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Proceedings of the National Academy of Sciences |
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105 |
container_issue |
6 |
container_start_page |
1993 |
op_container_end_page |
1998 |
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1766394478895038464 |