Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish
The antifreeze glycoprotein-fortified Antarctic notothenioid fishes comprise the predominant fish suborder in the isolated frigid Southern Ocean. Their ecological success undoubtedly entailed evolutionary acquisition of a full suite of cold-stable functions besides antifreeze protection. Prior studi...
Published in: | Proceedings of the National Academy of Sciences |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
National Academy of Sciences
2008
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529033 http://www.ncbi.nlm.nih.gov/pubmed/18753634 https://doi.org/10.1073/pnas.0802432105 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:2529033 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:2529033 2023-05-15T14:03:14+02:00 Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish Chen, Zuozhou Cheng, C.-H. Christina Zhang, Junfang Cao, Lixue Chen, Lei Zhou, Longhai Jin, Yudong Ye, Hua Deng, Cheng Dai, Zhonghua Xu, Qianghua Hu, Peng Sun, Shouhong Shen, Yu Chen, Liangbiao 2008-09-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529033 http://www.ncbi.nlm.nih.gov/pubmed/18753634 https://doi.org/10.1073/pnas.0802432105 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529033 http://www.ncbi.nlm.nih.gov/pubmed/18753634 http://dx.doi.org/10.1073/pnas.0802432105 © 2008 by The National Academy of Sciences of the USA Biological Sciences Text 2008 ftpubmed https://doi.org/10.1073/pnas.0802432105 2013-09-02T05:00:41Z The antifreeze glycoprotein-fortified Antarctic notothenioid fishes comprise the predominant fish suborder in the isolated frigid Southern Ocean. Their ecological success undoubtedly entailed evolutionary acquisition of a full suite of cold-stable functions besides antifreeze protection. Prior studies of adaptive changes in these teleost fishes generally examined a single genotype or phenotype. We report here the genome-wide investigations of transcriptional and genomic changes associated with Antarctic notothenioid cold adaptation. We sequenced and characterized 33,560 ESTs from four tissues of the Antarctic notothenioid Dissostichus mawsoni and derived 3,114 nonredundant protein gene families and their expression profiles. Through comparative analyses of same-tissue transcriptome profiles of D. mawsoni and temperate/tropical teleost fishes, we identified 177 notothenioid protein families that were expressed many fold over the latter, indicating cold-related up-regulation. These up-regulated gene families operate in protein biosynthesis, protein folding and degradation, lipid metabolism, antioxidation, antiapoptosis, innate immunity, choriongenesis, and others, all of recognizable functional importance in mitigating stresses in freezing temperatures during notothenioid life histories. We further examined the genomic and evolutionary bases for this expressional up-regulation by comparative genomic hybridization of DNA from four pairs of Antarctic and basal non-Antarctic notothenioids to 10,700 D. mawsoni cDNA probes and discovered significant to astounding (3- to >300-fold, P < 0.05) Antarctic-specific duplications of 118 protein-coding genes, many of which correspond to the up-regulated gene families. Results of our integrative tripartite study strongly suggest that evolution under constant cold has resulted in dramatic genomic expansions of specific protein gene families, augmenting gene expression and gene functions contributing to physiological fitness of Antarctic notothenioids in freezing polar ... Text Antarc* Antarctic Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean The Antarctic Proceedings of the National Academy of Sciences 105 35 12944 12949 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Biological Sciences |
spellingShingle |
Biological Sciences Chen, Zuozhou Cheng, C.-H. Christina Zhang, Junfang Cao, Lixue Chen, Lei Zhou, Longhai Jin, Yudong Ye, Hua Deng, Cheng Dai, Zhonghua Xu, Qianghua Hu, Peng Sun, Shouhong Shen, Yu Chen, Liangbiao Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
topic_facet |
Biological Sciences |
description |
The antifreeze glycoprotein-fortified Antarctic notothenioid fishes comprise the predominant fish suborder in the isolated frigid Southern Ocean. Their ecological success undoubtedly entailed evolutionary acquisition of a full suite of cold-stable functions besides antifreeze protection. Prior studies of adaptive changes in these teleost fishes generally examined a single genotype or phenotype. We report here the genome-wide investigations of transcriptional and genomic changes associated with Antarctic notothenioid cold adaptation. We sequenced and characterized 33,560 ESTs from four tissues of the Antarctic notothenioid Dissostichus mawsoni and derived 3,114 nonredundant protein gene families and their expression profiles. Through comparative analyses of same-tissue transcriptome profiles of D. mawsoni and temperate/tropical teleost fishes, we identified 177 notothenioid protein families that were expressed many fold over the latter, indicating cold-related up-regulation. These up-regulated gene families operate in protein biosynthesis, protein folding and degradation, lipid metabolism, antioxidation, antiapoptosis, innate immunity, choriongenesis, and others, all of recognizable functional importance in mitigating stresses in freezing temperatures during notothenioid life histories. We further examined the genomic and evolutionary bases for this expressional up-regulation by comparative genomic hybridization of DNA from four pairs of Antarctic and basal non-Antarctic notothenioids to 10,700 D. mawsoni cDNA probes and discovered significant to astounding (3- to >300-fold, P < 0.05) Antarctic-specific duplications of 118 protein-coding genes, many of which correspond to the up-regulated gene families. Results of our integrative tripartite study strongly suggest that evolution under constant cold has resulted in dramatic genomic expansions of specific protein gene families, augmenting gene expression and gene functions contributing to physiological fitness of Antarctic notothenioids in freezing polar ... |
format |
Text |
author |
Chen, Zuozhou Cheng, C.-H. Christina Zhang, Junfang Cao, Lixue Chen, Lei Zhou, Longhai Jin, Yudong Ye, Hua Deng, Cheng Dai, Zhonghua Xu, Qianghua Hu, Peng Sun, Shouhong Shen, Yu Chen, Liangbiao |
author_facet |
Chen, Zuozhou Cheng, C.-H. Christina Zhang, Junfang Cao, Lixue Chen, Lei Zhou, Longhai Jin, Yudong Ye, Hua Deng, Cheng Dai, Zhonghua Xu, Qianghua Hu, Peng Sun, Shouhong Shen, Yu Chen, Liangbiao |
author_sort |
Chen, Zuozhou |
title |
Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
title_short |
Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
title_full |
Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
title_fullStr |
Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
title_full_unstemmed |
Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish |
title_sort |
transcriptomic and genomic evolution under constant cold in antarctic notothenioid fish |
publisher |
National Academy of Sciences |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529033 http://www.ncbi.nlm.nih.gov/pubmed/18753634 https://doi.org/10.1073/pnas.0802432105 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2529033 http://www.ncbi.nlm.nih.gov/pubmed/18753634 http://dx.doi.org/10.1073/pnas.0802432105 |
op_rights |
© 2008 by The National Academy of Sciences of the USA |
op_doi |
https://doi.org/10.1073/pnas.0802432105 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
105 |
container_issue |
35 |
container_start_page |
12944 |
op_container_end_page |
12949 |
_version_ |
1766273790405246976 |