Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line
The organic solute and steroid transporter (OST/Ost) is a unique membrane transport protein heterodimer composed of subunits designated alpha and beta, that transports conjugated steroids and prostaglandin E2 across the plasma membrane. Ost was first identified in the liver of the cartilaginous fish...
Published in: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
2008
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471870 http://www.ncbi.nlm.nih.gov/pubmed/18407792 https://doi.org/10.1016/j.cbpc.2008.03.005 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:2471870 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:2471870 2023-05-15T18:51:08+02:00 Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line Hwang, Jae-Ho Parton, Angela Czechanski, Anne Ballatori, Nazzareno Barnes, David 2008-03-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471870 http://www.ncbi.nlm.nih.gov/pubmed/18407792 https://doi.org/10.1016/j.cbpc.2008.03.005 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471870 http://www.ncbi.nlm.nih.gov/pubmed/18407792 http://dx.doi.org/10.1016/j.cbpc.2008.03.005 Article Text 2008 ftpubmed https://doi.org/10.1016/j.cbpc.2008.03.005 2013-09-02T02:19:55Z The organic solute and steroid transporter (OST/Ost) is a unique membrane transport protein heterodimer composed of subunits designated alpha and beta, that transports conjugated steroids and prostaglandin E2 across the plasma membrane. Ost was first identified in the liver of the cartilaginous fish Leucoraja erinacea, the little skate, and subsequently was found in many other species, including humans and rodents. The present study describes the isolation of a new cell line, LEE-1, derived from an early embryo of L. erinacea, and characterizes the expression of Ost in these cells. The mRNA size and amino acid sequence of Ost-beta in LEE-1 was identical to that previously reported for Ost-beta from skate liver, and the primary structure was identical to that of the spiny dogfish shark (Squalus acanthias) with the exception of a single amino acid. Ost-beta was found both on the plasma membrane and intracellularly in LEE-1 cells, consistent with its localization in other cell types. Interestingly, arachidonic acid, the precursor to eiconsanoids, strongly induced Ost-beta expression in LEE-1 cells and a lipid mixture containing arachidonic acid also induced Ost-alpha. Overall, the present study describes the isolation of a novel marine cell line, and shows that this cell line expresses relatively high levels of Ost when cultured in the presence of arachidonic acid. Although the function of this transport protein in embryo-derived cells is unknown, it may play a role in the disposition of eicosanoids or steroid-derived molecules. Text spiny dogfish Squalus acanthias PubMed Central (PMC) Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 148 1 39 47 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Hwang, Jae-Ho Parton, Angela Czechanski, Anne Ballatori, Nazzareno Barnes, David Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
topic_facet |
Article |
description |
The organic solute and steroid transporter (OST/Ost) is a unique membrane transport protein heterodimer composed of subunits designated alpha and beta, that transports conjugated steroids and prostaglandin E2 across the plasma membrane. Ost was first identified in the liver of the cartilaginous fish Leucoraja erinacea, the little skate, and subsequently was found in many other species, including humans and rodents. The present study describes the isolation of a new cell line, LEE-1, derived from an early embryo of L. erinacea, and characterizes the expression of Ost in these cells. The mRNA size and amino acid sequence of Ost-beta in LEE-1 was identical to that previously reported for Ost-beta from skate liver, and the primary structure was identical to that of the spiny dogfish shark (Squalus acanthias) with the exception of a single amino acid. Ost-beta was found both on the plasma membrane and intracellularly in LEE-1 cells, consistent with its localization in other cell types. Interestingly, arachidonic acid, the precursor to eiconsanoids, strongly induced Ost-beta expression in LEE-1 cells and a lipid mixture containing arachidonic acid also induced Ost-alpha. Overall, the present study describes the isolation of a novel marine cell line, and shows that this cell line expresses relatively high levels of Ost when cultured in the presence of arachidonic acid. Although the function of this transport protein in embryo-derived cells is unknown, it may play a role in the disposition of eicosanoids or steroid-derived molecules. |
format |
Text |
author |
Hwang, Jae-Ho Parton, Angela Czechanski, Anne Ballatori, Nazzareno Barnes, David |
author_facet |
Hwang, Jae-Ho Parton, Angela Czechanski, Anne Ballatori, Nazzareno Barnes, David |
author_sort |
Hwang, Jae-Ho |
title |
Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
title_short |
Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
title_full |
Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
title_fullStr |
Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
title_full_unstemmed |
Arachidonic Acid-Induced Expression of the Organic Solute and Steroid Transporter-beta (Ost-beta) in a Cartilaginous Fish Cell Line |
title_sort |
arachidonic acid-induced expression of the organic solute and steroid transporter-beta (ost-beta) in a cartilaginous fish cell line |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471870 http://www.ncbi.nlm.nih.gov/pubmed/18407792 https://doi.org/10.1016/j.cbpc.2008.03.005 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471870 http://www.ncbi.nlm.nih.gov/pubmed/18407792 http://dx.doi.org/10.1016/j.cbpc.2008.03.005 |
op_doi |
https://doi.org/10.1016/j.cbpc.2008.03.005 |
container_title |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
container_volume |
148 |
container_issue |
1 |
container_start_page |
39 |
op_container_end_page |
47 |
_version_ |
1766244920795856896 |