Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitatio...
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ftpubmed:oai:pubmedcentral.nih.gov:243545 2023-05-15T15:58:05+02:00 Modified procedure for the recovery of naturally accumulated poliovirus from oysters. Vaughn, J M Landry, E F Vicale, T J Dahl, M C 1979-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 Research Article Text 1979 ftpubmed 2013-08-29T16:07:27Z Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters. Text Crassostrea gigas PubMed Central (PMC) |
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English |
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Research Article |
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Research Article Vaughn, J M Landry, E F Vicale, T J Dahl, M C Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
topic_facet |
Research Article |
description |
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters. |
format |
Text |
author |
Vaughn, J M Landry, E F Vicale, T J Dahl, M C |
author_facet |
Vaughn, J M Landry, E F Vicale, T J Dahl, M C |
author_sort |
Vaughn, J M |
title |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
title_short |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
title_full |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
title_fullStr |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
title_full_unstemmed |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
title_sort |
modified procedure for the recovery of naturally accumulated poliovirus from oysters. |
publishDate |
1979 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 |
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1766393804604047360 |