Modified procedure for the recovery of naturally accumulated poliovirus from oysters.

Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitatio...

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Main Authors: Vaughn, J M, Landry, E F, Vicale, T J, Dahl, M C
Format: Text
Language:English
Published: 1979
Subjects:
Research Article
Crassostrea gigas
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545
http://www.ncbi.nlm.nih.gov/pubmed/231934
id ftpubmed:oai:pubmedcentral.nih.gov:243545
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:243545 2023-05-15T15:58:05+02:00 Modified procedure for the recovery of naturally accumulated poliovirus from oysters. Vaughn, J M Landry, E F Vicale, T J Dahl, M C 1979-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545 http://www.ncbi.nlm.nih.gov/pubmed/231934 Research Article Text 1979 ftpubmed 2013-08-29T16:07:27Z Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters. Text Crassostrea gigas PubMed Central (PMC)
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Research Article
spellingShingle Research Article
Vaughn, J M
Landry, E F
Vicale, T J
Dahl, M C
Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
topic_facet Research Article
description Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters.
format Text
author Vaughn, J M
Landry, E F
Vicale, T J
Dahl, M C
author_facet Vaughn, J M
Landry, E F
Vicale, T J
Dahl, M C
author_sort Vaughn, J M
title Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
title_short Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
title_full Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
title_fullStr Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
title_full_unstemmed Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
title_sort modified procedure for the recovery of naturally accumulated poliovirus from oysters.
publishDate 1979
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545
http://www.ncbi.nlm.nih.gov/pubmed/231934
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243545
http://www.ncbi.nlm.nih.gov/pubmed/231934
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