Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ †
Understanding the factors that influence the distribution and abundance of marine diazotrophs is important in order to assess their role in the oceanic nitrogen cycle. Environmental DNA samples from four cruises to the North Atlantic Ocean, covering a sampling area of 0°N to 42°N and 67°W to 13°W, w...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology (ASM)
2008
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268318 http://www.ncbi.nlm.nih.gov/pubmed/18245263 https://doi.org/10.1128/AEM.01720-07 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:2268318 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:2268318 2023-05-15T17:34:56+02:00 Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † Langlois, Rebecca J. Hümmer, Diana LaRoche, Julie 2008-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268318 http://www.ncbi.nlm.nih.gov/pubmed/18245263 https://doi.org/10.1128/AEM.01720-07 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268318 http://www.ncbi.nlm.nih.gov/pubmed/18245263 http://dx.doi.org/10.1128/AEM.01720-07 Copyright © 2008, American Society for Microbiology Microbial Ecology Text 2008 ftpubmed https://doi.org/10.1128/AEM.01720-07 2013-09-01T16:32:08Z Understanding the factors that influence the distribution and abundance of marine diazotrophs is important in order to assess their role in the oceanic nitrogen cycle. Environmental DNA samples from four cruises to the North Atlantic Ocean, covering a sampling area of 0°N to 42°N and 67°W to 13°W, were analyzed for the presence and amount of seven nifH phylotypes using real-time quantitative PCR and TaqMan probes. The cyanobacterial phylotypes dominated in abundance (94% of all nifH copies detected) and were the most widely distributed. The filamentous cyanobacterial type, which included both Trichodesmium and Katagnymene, was the most abundant (51%), followed by group A, an uncultured unicellular cyanobacterium (33%), and gamma A, an uncultured gammaproteobacterium (6%). Group B, unicellular cyanobacterium Crocosphaera, and group C Cyanothece-like phylotypes were not often detected (6.9% and 2.3%, respectively), but where present, could reach high concentrations. Gamma P, another uncultured gammaproteobacterium, was seldom detected (0.5%). Water temperature appeared to influence the distribution of many nifH phylotypes. Very high (up to 1 × 106 copies liter−1) nifH concentrations of group A were detected in the eastern basin (25 to 17°N, 27 to 30°W), where the temperature ranged from 20 to 23°C. The highest concentrations of filamentous phylotypes were measured between 25 and 30°C. The uncultured cluster III phylotype was uncommon (0.4%) and was associated with mean water temperatures of 18°C. Diazotroph abundance was highest in regions where modeled average dust deposition was between 1 and 2 g/m2/year. Text North Atlantic PubMed Central (PMC) Applied and Environmental Microbiology 74 6 1922 1931 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Langlois, Rebecca J. Hümmer, Diana LaRoche, Julie Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
topic_facet |
Microbial Ecology |
description |
Understanding the factors that influence the distribution and abundance of marine diazotrophs is important in order to assess their role in the oceanic nitrogen cycle. Environmental DNA samples from four cruises to the North Atlantic Ocean, covering a sampling area of 0°N to 42°N and 67°W to 13°W, were analyzed for the presence and amount of seven nifH phylotypes using real-time quantitative PCR and TaqMan probes. The cyanobacterial phylotypes dominated in abundance (94% of all nifH copies detected) and were the most widely distributed. The filamentous cyanobacterial type, which included both Trichodesmium and Katagnymene, was the most abundant (51%), followed by group A, an uncultured unicellular cyanobacterium (33%), and gamma A, an uncultured gammaproteobacterium (6%). Group B, unicellular cyanobacterium Crocosphaera, and group C Cyanothece-like phylotypes were not often detected (6.9% and 2.3%, respectively), but where present, could reach high concentrations. Gamma P, another uncultured gammaproteobacterium, was seldom detected (0.5%). Water temperature appeared to influence the distribution of many nifH phylotypes. Very high (up to 1 × 106 copies liter−1) nifH concentrations of group A were detected in the eastern basin (25 to 17°N, 27 to 30°W), where the temperature ranged from 20 to 23°C. The highest concentrations of filamentous phylotypes were measured between 25 and 30°C. The uncultured cluster III phylotype was uncommon (0.4%) and was associated with mean water temperatures of 18°C. Diazotroph abundance was highest in regions where modeled average dust deposition was between 1 and 2 g/m2/year. |
format |
Text |
author |
Langlois, Rebecca J. Hümmer, Diana LaRoche, Julie |
author_facet |
Langlois, Rebecca J. Hümmer, Diana LaRoche, Julie |
author_sort |
Langlois, Rebecca J. |
title |
Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
title_short |
Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
title_full |
Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
title_fullStr |
Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
title_full_unstemmed |
Abundances and Distributions of the Dominant nifH Phylotypes in the Northern Atlantic Ocean▿ † |
title_sort |
abundances and distributions of the dominant nifh phylotypes in the northern atlantic ocean▿ † |
publisher |
American Society for Microbiology (ASM) |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268318 http://www.ncbi.nlm.nih.gov/pubmed/18245263 https://doi.org/10.1128/AEM.01720-07 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268318 http://www.ncbi.nlm.nih.gov/pubmed/18245263 http://dx.doi.org/10.1128/AEM.01720-07 |
op_rights |
Copyright © 2008, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.01720-07 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
6 |
container_start_page |
1922 |
op_container_end_page |
1931 |
_version_ |
1766133938661621760 |