T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and ma...
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ftpubmed:oai:pubmedcentral.nih.gov:2213496 2023-05-15T18:26:47+02:00 T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. 2000-12-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213496 http://www.ncbi.nlm.nih.gov/pubmed/11120769 en eng The Rockefeller University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213496 http://www.ncbi.nlm.nih.gov/pubmed/11120769 © 2000 The Rockefeller University Press Original Article Text 2000 ftpubmed 2013-09-01T13:51:51Z We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4+ T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4+ T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4+ T cell memory. Text Sperm whale PubMed Central (PMC) |
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Original Article Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
topic_facet |
Original Article |
description |
We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4+ T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4+ T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4+ T cell memory. |
format |
Text |
author |
Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. |
author_facet |
Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. |
author_sort |
Fassò, Marcella |
title |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_short |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_full |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_fullStr |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_full_unstemmed |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_sort |
t cell receptor (tcr)-mediated repertoire selection and loss of tcr vβ diversity during the initiation of a cd4+ t cell response in vivo |
publisher |
The Rockefeller University Press |
publishDate |
2000 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213496 http://www.ncbi.nlm.nih.gov/pubmed/11120769 |
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Sperm whale |
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Sperm whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213496 http://www.ncbi.nlm.nih.gov/pubmed/11120769 |
op_rights |
© 2000 The Rockefeller University Press |
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