Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa.
A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2(1)2(1)2(1). A complete data set to 2.1 A resolution has been measured....
Main Authors: | , , , |
---|---|
Format: | Text |
Language: | English |
Published: |
Cold Spring Harbor Laboratory Press
1997
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143593 http://www.ncbi.nlm.nih.gov/pubmed/9385650 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:2143593 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:2143593 2023-05-15T13:38:05+02:00 Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. Villeret, V. Chessa, J. P. Gerday, C. Van Beeumen, J. 1997-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143593 http://www.ncbi.nlm.nih.gov/pubmed/9385650 en eng Cold Spring Harbor Laboratory Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143593 http://www.ncbi.nlm.nih.gov/pubmed/9385650 Research Article Text 1997 ftpubmed 2013-09-01T10:28:50Z A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2(1)2(1)2(1). A complete data set to 2.1 A resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R-factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an alpha-amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Villeret, V. Chessa, J. P. Gerday, C. Van Beeumen, J. Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
topic_facet |
Research Article |
description |
A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2(1)2(1)2(1). A complete data set to 2.1 A resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R-factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an alpha-amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme. |
format |
Text |
author |
Villeret, V. Chessa, J. P. Gerday, C. Van Beeumen, J. |
author_facet |
Villeret, V. Chessa, J. P. Gerday, C. Van Beeumen, J. |
author_sort |
Villeret, V. |
title |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
title_short |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
title_full |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
title_fullStr |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
title_full_unstemmed |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. |
title_sort |
preliminary crystal structure determination of the alkaline protease from the antarctic psychrophile pseudomonas aeruginosa. |
publisher |
Cold Spring Harbor Laboratory Press |
publishDate |
1997 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143593 http://www.ncbi.nlm.nih.gov/pubmed/9385650 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143593 http://www.ncbi.nlm.nih.gov/pubmed/9385650 |
_version_ |
1766101430042624000 |