The T cell receptor repertoire influences V beta element usage in response to myoglobin
T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110- 121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) V beta usage in DBA/2 mice. All clones previously tested used the same V beta 8.2 ge...
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ftpubmed:oai:pubmedcentral.nih.gov:2118891 2023-05-15T18:26:49+02:00 The T cell receptor repertoire influences V beta element usage in response to myoglobin 1991-07-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118891 http://www.ncbi.nlm.nih.gov/pubmed/2056283 en eng The Rockefeller University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118891 http://www.ncbi.nlm.nih.gov/pubmed/2056283 Articles Text 1991 ftpubmed 2013-09-01T09:12:58Z T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110- 121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) V beta usage in DBA/2 mice. All clones previously tested used the same V beta 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpW Mb protein or peptide 110-121. Only the V beta 8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V beta genes, lacked V beta 8+ peripheral T cells. DBA/2 mice depleted of V beta 8+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2V beta a mice (homozygous for a deletion of multiple V beta gene segments including the V beta 8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V beta a mice had a very restricted TCR V beta repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2V beta a and F23.1-treated DBA/2 mice are discussed. Text Sperm whale PubMed Central (PMC) |
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Articles The T cell receptor repertoire influences V beta element usage in response to myoglobin |
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T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110- 121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) V beta usage in DBA/2 mice. All clones previously tested used the same V beta 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpW Mb protein or peptide 110-121. Only the V beta 8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V beta genes, lacked V beta 8+ peripheral T cells. DBA/2 mice depleted of V beta 8+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2V beta a mice (homozygous for a deletion of multiple V beta gene segments including the V beta 8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V beta a mice had a very restricted TCR V beta repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2V beta a and F23.1-treated DBA/2 mice are discussed. |
format |
Text |
title |
The T cell receptor repertoire influences V beta element usage in response to myoglobin |
title_short |
The T cell receptor repertoire influences V beta element usage in response to myoglobin |
title_full |
The T cell receptor repertoire influences V beta element usage in response to myoglobin |
title_fullStr |
The T cell receptor repertoire influences V beta element usage in response to myoglobin |
title_full_unstemmed |
The T cell receptor repertoire influences V beta element usage in response to myoglobin |
title_sort |
t cell receptor repertoire influences v beta element usage in response to myoglobin |
publisher |
The Rockefeller University Press |
publishDate |
1991 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118891 http://www.ncbi.nlm.nih.gov/pubmed/2056283 |
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Sperm whale |
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Sperm whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118891 http://www.ncbi.nlm.nih.gov/pubmed/2056283 |
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1766208792343609344 |