Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI
A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LPS, to display bactericidal activity against Escherichia coli, and to increas...
Published in: | Proceedings of the National Academy of Sciences |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
National Academy of Sciences
2007
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077063 http://www.ncbi.nlm.nih.gov/pubmed/17965238 https://doi.org/10.1073/pnas.0702281104 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:2077063 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:2077063 2023-05-15T15:57:48+02:00 Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI Gonzalez, Marcelo Gueguen, Yannick Destoumieux-Garzón, Delphine Romestand, Bernard Fievet, Julie Pugnière, Martine Roquet, Françoise Escoubas, Jean-Michel Vandenbulcke, Franck Levy, Ofer Sauné, Laure Bulet, Philippe Bachère, Evelyne 2007-11-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077063 http://www.ncbi.nlm.nih.gov/pubmed/17965238 https://doi.org/10.1073/pnas.0702281104 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077063 http://www.ncbi.nlm.nih.gov/pubmed/17965238 http://dx.doi.org/10.1073/pnas.0702281104 © 2007 by The National Academy of Sciences of the USA Biological Sciences Text 2007 ftpubmed https://doi.org/10.1073/pnas.0702281104 2013-09-01T07:10:02Z A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C. gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). Therefore, Cg-BPI, whose expression profile and biological properties are reminiscent of mammalian BPIs, may provide a first line of defense against potential bacterial invasion. To our knowledge, this is the first characterization of a BPI in an invertebrate. Text Crassostrea gigas PubMed Central (PMC) Proceedings of the National Academy of Sciences 104 45 17759 17764 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Biological Sciences |
spellingShingle |
Biological Sciences Gonzalez, Marcelo Gueguen, Yannick Destoumieux-Garzón, Delphine Romestand, Bernard Fievet, Julie Pugnière, Martine Roquet, Françoise Escoubas, Jean-Michel Vandenbulcke, Franck Levy, Ofer Sauné, Laure Bulet, Philippe Bachère, Evelyne Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
topic_facet |
Biological Sciences |
description |
A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C. gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). Therefore, Cg-BPI, whose expression profile and biological properties are reminiscent of mammalian BPIs, may provide a first line of defense against potential bacterial invasion. To our knowledge, this is the first characterization of a BPI in an invertebrate. |
format |
Text |
author |
Gonzalez, Marcelo Gueguen, Yannick Destoumieux-Garzón, Delphine Romestand, Bernard Fievet, Julie Pugnière, Martine Roquet, Françoise Escoubas, Jean-Michel Vandenbulcke, Franck Levy, Ofer Sauné, Laure Bulet, Philippe Bachère, Evelyne |
author_facet |
Gonzalez, Marcelo Gueguen, Yannick Destoumieux-Garzón, Delphine Romestand, Bernard Fievet, Julie Pugnière, Martine Roquet, Françoise Escoubas, Jean-Michel Vandenbulcke, Franck Levy, Ofer Sauné, Laure Bulet, Philippe Bachère, Evelyne |
author_sort |
Gonzalez, Marcelo |
title |
Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
title_short |
Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
title_full |
Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
title_fullStr |
Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
title_full_unstemmed |
Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI |
title_sort |
evidence of a bactericidal permeability increasing protein in an invertebrate, the crassostrea gigas cg-bpi |
publisher |
National Academy of Sciences |
publishDate |
2007 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077063 http://www.ncbi.nlm.nih.gov/pubmed/17965238 https://doi.org/10.1073/pnas.0702281104 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077063 http://www.ncbi.nlm.nih.gov/pubmed/17965238 http://dx.doi.org/10.1073/pnas.0702281104 |
op_rights |
© 2007 by The National Academy of Sciences of the USA |
op_doi |
https://doi.org/10.1073/pnas.0702281104 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
104 |
container_issue |
45 |
container_start_page |
17759 |
op_container_end_page |
17764 |
_version_ |
1766393492670513152 |