Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿
Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant througho...
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American Society for Microbiology
2007
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828651 http://www.ncbi.nlm.nih.gov/pubmed/17189433 https://doi.org/10.1128/AEM.02243-06 |
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ftpubmed:oai:pubmedcentral.nih.gov:1828651 2023-05-15T18:41:15+02:00 Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng 2007-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828651 http://www.ncbi.nlm.nih.gov/pubmed/17189433 https://doi.org/10.1128/AEM.02243-06 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828651 http://www.ncbi.nlm.nih.gov/pubmed/17189433 http://dx.doi.org/10.1128/AEM.02243-06 Copyright © 2007, American Society for Microbiology Microbial Ecology Text 2007 ftpubmed https://doi.org/10.1128/AEM.02243-06 2013-08-31T19:03:42Z Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 109 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity. Text Turbot PubMed Central (PMC) Applied and Environmental Microbiology 73 4 1349 1354 |
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Microbial Ecology |
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Microbial Ecology Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
topic_facet |
Microbial Ecology |
description |
Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 109 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity. |
format |
Text |
author |
Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng |
author_facet |
Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng |
author_sort |
Du, Meng |
title |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
title_short |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
title_full |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
title_fullStr |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
title_full_unstemmed |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate▿ |
title_sort |
retention of virulence in a viable but nonculturable edwardsiella tarda isolate▿ |
publisher |
American Society for Microbiology |
publishDate |
2007 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828651 http://www.ncbi.nlm.nih.gov/pubmed/17189433 https://doi.org/10.1128/AEM.02243-06 |
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Turbot |
genre_facet |
Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828651 http://www.ncbi.nlm.nih.gov/pubmed/17189433 http://dx.doi.org/10.1128/AEM.02243-06 |
op_rights |
Copyright © 2007, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.02243-06 |
container_title |
Applied and Environmental Microbiology |
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73 |
container_issue |
4 |
container_start_page |
1349 |
op_container_end_page |
1354 |
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1766230740146585600 |