An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus)
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed again...
Published in: | Acta Veterinaria Scandinavica |
---|---|
Main Author: | |
Format: | Text |
Language: | English |
Published: |
BioMed Central
2002
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764197 http://www.ncbi.nlm.nih.gov/pubmed/12831174 https://doi.org/10.1186/1751-0147-43-211 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:1764197 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:1764197 2023-05-15T13:19:45+02:00 An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) Åkerstedt, J 2002 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764197 http://www.ncbi.nlm.nih.gov/pubmed/12831174 https://doi.org/10.1186/1751-0147-43-211 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764197 http://www.ncbi.nlm.nih.gov/pubmed/12831174 http://dx.doi.org/10.1186/1751-0147-43-211 Original Article Text 2002 ftpubmed https://doi.org/10.1186/1751-0147-43-211 2013-08-31T15:59:57Z Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. Text Alopex lagopus PubMed Central (PMC) Acta Veterinaria Scandinavica 43 4 211 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Article |
spellingShingle |
Original Article Åkerstedt, J An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
topic_facet |
Original Article |
description |
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. |
format |
Text |
author |
Åkerstedt, J |
author_facet |
Åkerstedt, J |
author_sort |
Åkerstedt, J |
title |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_short |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_fullStr |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full_unstemmed |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_sort |
indirect elisa for detection of encephalitozoon cuniculi infection in farmed blue foxes (alopex lagopus) |
publisher |
BioMed Central |
publishDate |
2002 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764197 http://www.ncbi.nlm.nih.gov/pubmed/12831174 https://doi.org/10.1186/1751-0147-43-211 |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764197 http://www.ncbi.nlm.nih.gov/pubmed/12831174 http://dx.doi.org/10.1186/1751-0147-43-211 |
op_doi |
https://doi.org/10.1186/1751-0147-43-211 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
43 |
container_issue |
4 |
container_start_page |
211 |
_version_ |
1766349389000867840 |