Protein preservation and DNA retrieval from ancient tissues
The retrieval of DNA from fossils remains controversial. To substantiate claims of DNA recovery, one needs additional information on the preservation of other molecules within the same sample. Flash pyrolysis with GC and MS was used to assess the quality of protein preservation in 11 archaeological...
Main Authors: | , |
---|---|
Format: | Text |
Language: | English |
Published: |
The National Academy of Sciences
1999
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC17532 http://www.ncbi.nlm.nih.gov/pubmed/10411891 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:17532 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:17532 2023-05-15T15:04:06+02:00 Protein preservation and DNA retrieval from ancient tissues Poinar, Hendrik N. Stankiewicz, B. Artur 1999-07-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC17532 http://www.ncbi.nlm.nih.gov/pubmed/10411891 en eng The National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC17532 http://www.ncbi.nlm.nih.gov/pubmed/10411891 Copyright © 1999, The National Academy of Sciences Biological Sciences Text 1999 ftpubmed 2013-08-29T06:49:29Z The retrieval of DNA from fossils remains controversial. To substantiate claims of DNA recovery, one needs additional information on the preservation of other molecules within the same sample. Flash pyrolysis with GC and MS was used to assess the quality of protein preservation in 11 archaeological and paleontological remains, some of which have yielded ancient DNA sequences authenticated via a number of criteria and some of which have consistently failed to yield any meaningful DNA. Several samples, including the Neanderthal-type specimen from which DNA sequences were recently reported, yielded abundant pyrolysis products assigned to 2,5-diketopiperazines of proline-containing dipeptides. The relative amounts of these products provide a good index of the amount of peptide hydrolysis and DNA preservation. Of these samples, four stem from arctic or subarctic regions, emphasizing the importance of cooler temperatures for the preservation of macromolecules. Flash pyrolysis with GC and MS offers a rapid and effective method for assessing fossils for the possibility of DNA preservation. Text Arctic Subarctic PubMed Central (PMC) Arctic |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Biological Sciences |
spellingShingle |
Biological Sciences Poinar, Hendrik N. Stankiewicz, B. Artur Protein preservation and DNA retrieval from ancient tissues |
topic_facet |
Biological Sciences |
description |
The retrieval of DNA from fossils remains controversial. To substantiate claims of DNA recovery, one needs additional information on the preservation of other molecules within the same sample. Flash pyrolysis with GC and MS was used to assess the quality of protein preservation in 11 archaeological and paleontological remains, some of which have yielded ancient DNA sequences authenticated via a number of criteria and some of which have consistently failed to yield any meaningful DNA. Several samples, including the Neanderthal-type specimen from which DNA sequences were recently reported, yielded abundant pyrolysis products assigned to 2,5-diketopiperazines of proline-containing dipeptides. The relative amounts of these products provide a good index of the amount of peptide hydrolysis and DNA preservation. Of these samples, four stem from arctic or subarctic regions, emphasizing the importance of cooler temperatures for the preservation of macromolecules. Flash pyrolysis with GC and MS offers a rapid and effective method for assessing fossils for the possibility of DNA preservation. |
format |
Text |
author |
Poinar, Hendrik N. Stankiewicz, B. Artur |
author_facet |
Poinar, Hendrik N. Stankiewicz, B. Artur |
author_sort |
Poinar, Hendrik N. |
title |
Protein preservation and DNA retrieval from ancient tissues |
title_short |
Protein preservation and DNA retrieval from ancient tissues |
title_full |
Protein preservation and DNA retrieval from ancient tissues |
title_fullStr |
Protein preservation and DNA retrieval from ancient tissues |
title_full_unstemmed |
Protein preservation and DNA retrieval from ancient tissues |
title_sort |
protein preservation and dna retrieval from ancient tissues |
publisher |
The National Academy of Sciences |
publishDate |
1999 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC17532 http://www.ncbi.nlm.nih.gov/pubmed/10411891 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Subarctic |
genre_facet |
Arctic Subarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC17532 http://www.ncbi.nlm.nih.gov/pubmed/10411891 |
op_rights |
Copyright © 1999, The National Academy of Sciences |
_version_ |
1766335914712236032 |