Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿
Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most clo...
Published in: | Journal of Clinical Microbiology |
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ftpubmed:oai:pubmedcentral.nih.gov:1698438 2023-05-15T17:58:55+02:00 Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ McDonald, W. L. Jamaludin, R. Mackereth, G. Hansen, M. Humphrey, S. Short, P. Taylor, T. Swingler, J. Dawson, C. E. Whatmore, A. M. Stubberfield, E. Perrett, L. L. Simmons, G. 2006-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698438 http://www.ncbi.nlm.nih.gov/pubmed/17035490 https://doi.org/10.1128/JCM.00680-06 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698438 http://www.ncbi.nlm.nih.gov/pubmed/17035490 http://dx.doi.org/10.1128/JCM.00680-06 Copyright © 2006, American Society for Microbiology Bacteriology Text 2006 ftpubmed https://doi.org/10.1128/JCM.00680-06 2013-08-31T12:54:18Z Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to strains previously identified from marine mammals. The isolate was preliminarily identified as Brucella suis using conventional bacteriological tests in our laboratory. However, the results profile was not an exact match, and the isolate was forwarded to four international reference laboratories for further identification. The reference laboratories identified the isolate as either B. suis or B. melitensis by traditional bacteriological methods in three laboratories and by a molecular test in the fourth laboratory. Molecular characterization by PCR, PCR-restriction fragment length polymorphism, and DNA sequencing of the bp26 gene; IS711; the omp genes omp25, omp31, omp2a, and omp2b; IRS-PCR fragments I, III, and IV; and five housekeeping gene fragments was conducted to resolve the discrepant identification of the isolate. The isolate was identified to be closely related to a Brucella sp. originating from a United States bottlenose dolphin (Tursiops truncatus) and common seals (Phoca vitulina). Text Phoca vitulina PubMed Central (PMC) New Zealand Journal of Clinical Microbiology 44 12 4363 4370 |
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Bacteriology |
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Bacteriology McDonald, W. L. Jamaludin, R. Mackereth, G. Hansen, M. Humphrey, S. Short, P. Taylor, T. Swingler, J. Dawson, C. E. Whatmore, A. M. Stubberfield, E. Perrett, L. L. Simmons, G. Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
topic_facet |
Bacteriology |
description |
Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to strains previously identified from marine mammals. The isolate was preliminarily identified as Brucella suis using conventional bacteriological tests in our laboratory. However, the results profile was not an exact match, and the isolate was forwarded to four international reference laboratories for further identification. The reference laboratories identified the isolate as either B. suis or B. melitensis by traditional bacteriological methods in three laboratories and by a molecular test in the fourth laboratory. Molecular characterization by PCR, PCR-restriction fragment length polymorphism, and DNA sequencing of the bp26 gene; IS711; the omp genes omp25, omp31, omp2a, and omp2b; IRS-PCR fragments I, III, and IV; and five housekeeping gene fragments was conducted to resolve the discrepant identification of the isolate. The isolate was identified to be closely related to a Brucella sp. originating from a United States bottlenose dolphin (Tursiops truncatus) and common seals (Phoca vitulina). |
format |
Text |
author |
McDonald, W. L. Jamaludin, R. Mackereth, G. Hansen, M. Humphrey, S. Short, P. Taylor, T. Swingler, J. Dawson, C. E. Whatmore, A. M. Stubberfield, E. Perrett, L. L. Simmons, G. |
author_facet |
McDonald, W. L. Jamaludin, R. Mackereth, G. Hansen, M. Humphrey, S. Short, P. Taylor, T. Swingler, J. Dawson, C. E. Whatmore, A. M. Stubberfield, E. Perrett, L. L. Simmons, G. |
author_sort |
McDonald, W. L. |
title |
Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
title_short |
Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
title_full |
Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
title_fullStr |
Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
title_full_unstemmed |
Characterization of a Brucella sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand▿ |
title_sort |
characterization of a brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in new zealand▿ |
publisher |
American Society for Microbiology |
publishDate |
2006 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698438 http://www.ncbi.nlm.nih.gov/pubmed/17035490 https://doi.org/10.1128/JCM.00680-06 |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698438 http://www.ncbi.nlm.nih.gov/pubmed/17035490 http://dx.doi.org/10.1128/JCM.00680-06 |
op_rights |
Copyright © 2006, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/JCM.00680-06 |
container_title |
Journal of Clinical Microbiology |
container_volume |
44 |
container_issue |
12 |
container_start_page |
4363 |
op_container_end_page |
4370 |
_version_ |
1766167649078738944 |