Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments
The distribution and activity of communities of sulfate-reducing bacteria (SRB) and methanogenic archaea in two contrasting Antarctic sediments were investigated. Methanogenesis dominated in freshwater Lake Heywood, while sulfate reduction dominated in marine Shallow Bay. Slurry experiments indicate...
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ftpubmed:oai:pubmedcentral.nih.gov:161550 2023-05-15T14:01:01+02:00 Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments Purdy, K. J. Nedwell, D. B. Embley, T. M. 2003-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161550 http://www.ncbi.nlm.nih.gov/pubmed/12788715 https://doi.org/10.1128/AEM.69.6.3181-3191.2003 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161550 http://www.ncbi.nlm.nih.gov/pubmed/12788715 http://dx.doi.org/10.1128/AEM.69.6.3181-3191.2003 Copyright © 2003, American Society for Microbiology Microbial Ecology Text 2003 ftpubmed https://doi.org/10.1128/AEM.69.6.3181-3191.2003 2013-08-29T12:21:09Z The distribution and activity of communities of sulfate-reducing bacteria (SRB) and methanogenic archaea in two contrasting Antarctic sediments were investigated. Methanogenesis dominated in freshwater Lake Heywood, while sulfate reduction dominated in marine Shallow Bay. Slurry experiments indicated that 90% of the methanogenesis in Lake Heywood was acetoclastic. This finding was supported by the limited diversity of clones detected in a Lake Heywood archaeal clone library, in which most clones were closely related to the obligate acetate-utilizing Methanosaeta concilii. The Shallow Bay archaeal clone library contained clones related to the C1-utilizing Methanolobus and Methanococcoides and the H2-utilizing Methanogenium. Oligonucleotide probing of RNA extracted directly from sediment indicated that archaea represented 34% of the total prokaryotic signal in Lake Heywood and that Methanosaeta was a major component (13.2%) of this signal. Archaea represented only 0.2% of the total prokaryotic signal in RNA extracted from Shallow Bay sediments. In the Shallow Bay bacterial clone library, 10.3% of the clones were SRB-like, related to Desulfotalea/Desulforhopalus, Desulfofaba, Desulfosarcina, and Desulfobacter as well as to the sulfur and metal oxidizers comprising the Desulfuromonas cluster. Oligonucleotide probes for specific SRB clusters indicated that SRB represented 14.7% of the total prokaryotic signal, with Desulfotalea/Desulforhopalus being the dominant SRB group (10.7% of the total prokaryotic signal) in the Shallow Bay sediments; these results support previous results obtained for Arctic sediments. Methanosaeta and Desulfotalea/Desulforhopalus appear to be important in Lake Heywood and Shallow Bay, respectively, and may be globally important in permanently low-temperature sediments. Text Antarc* Antarctic Arctic PubMed Central (PMC) Antarctic Arctic Heywood ENVELOPE(-59.683,-59.683,-62.317,-62.317) Shallow Bay ENVELOPE(67.467,67.467,-67.817,-67.817) Applied and Environmental Microbiology 69 6 3181 3191 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Purdy, K. J. Nedwell, D. B. Embley, T. M. Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
topic_facet |
Microbial Ecology |
description |
The distribution and activity of communities of sulfate-reducing bacteria (SRB) and methanogenic archaea in two contrasting Antarctic sediments were investigated. Methanogenesis dominated in freshwater Lake Heywood, while sulfate reduction dominated in marine Shallow Bay. Slurry experiments indicated that 90% of the methanogenesis in Lake Heywood was acetoclastic. This finding was supported by the limited diversity of clones detected in a Lake Heywood archaeal clone library, in which most clones were closely related to the obligate acetate-utilizing Methanosaeta concilii. The Shallow Bay archaeal clone library contained clones related to the C1-utilizing Methanolobus and Methanococcoides and the H2-utilizing Methanogenium. Oligonucleotide probing of RNA extracted directly from sediment indicated that archaea represented 34% of the total prokaryotic signal in Lake Heywood and that Methanosaeta was a major component (13.2%) of this signal. Archaea represented only 0.2% of the total prokaryotic signal in RNA extracted from Shallow Bay sediments. In the Shallow Bay bacterial clone library, 10.3% of the clones were SRB-like, related to Desulfotalea/Desulforhopalus, Desulfofaba, Desulfosarcina, and Desulfobacter as well as to the sulfur and metal oxidizers comprising the Desulfuromonas cluster. Oligonucleotide probes for specific SRB clusters indicated that SRB represented 14.7% of the total prokaryotic signal, with Desulfotalea/Desulforhopalus being the dominant SRB group (10.7% of the total prokaryotic signal) in the Shallow Bay sediments; these results support previous results obtained for Arctic sediments. Methanosaeta and Desulfotalea/Desulforhopalus appear to be important in Lake Heywood and Shallow Bay, respectively, and may be globally important in permanently low-temperature sediments. |
format |
Text |
author |
Purdy, K. J. Nedwell, D. B. Embley, T. M. |
author_facet |
Purdy, K. J. Nedwell, D. B. Embley, T. M. |
author_sort |
Purdy, K. J. |
title |
Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
title_short |
Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
title_full |
Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
title_fullStr |
Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
title_full_unstemmed |
Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments |
title_sort |
analysis of the sulfate-reducing bacterial and methanogenic archaeal populations in contrasting antarctic sediments |
publisher |
American Society for Microbiology |
publishDate |
2003 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161550 http://www.ncbi.nlm.nih.gov/pubmed/12788715 https://doi.org/10.1128/AEM.69.6.3181-3191.2003 |
long_lat |
ENVELOPE(-59.683,-59.683,-62.317,-62.317) ENVELOPE(67.467,67.467,-67.817,-67.817) |
geographic |
Antarctic Arctic Heywood Shallow Bay |
geographic_facet |
Antarctic Arctic Heywood Shallow Bay |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161550 http://www.ncbi.nlm.nih.gov/pubmed/12788715 http://dx.doi.org/10.1128/AEM.69.6.3181-3191.2003 |
op_rights |
Copyright © 2003, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.69.6.3181-3191.2003 |
container_title |
Applied and Environmental Microbiology |
container_volume |
69 |
container_issue |
6 |
container_start_page |
3181 |
op_container_end_page |
3191 |
_version_ |
1766270456164253696 |