Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source
Seasonal shifts in bacterioplankton community composition in Toolik Lake, a tundra lake on the North Slope of Alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (DOM). A shift in community composition, measured by denaturing gradi...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
2003
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ftpubmed:oai:pubmedcentral.nih.gov:154827 2023-05-15T15:08:57+02:00 Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source Crump, Byron C. Kling, George W. Bahr, Michele Hobbie, John E. 2003-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154827 http://www.ncbi.nlm.nih.gov/pubmed/12676708 https://doi.org/10.1128/AEM.69.4.2253-2268.2003 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154827 http://www.ncbi.nlm.nih.gov/pubmed/12676708 http://dx.doi.org/10.1128/AEM.69.4.2253-2268.2003 Copyright © 2003, American Society for Microbiology Microbial Ecology Text 2003 ftpubmed https://doi.org/10.1128/AEM.69.4.2253-2268.2003 2013-08-29T12:02:10Z Seasonal shifts in bacterioplankton community composition in Toolik Lake, a tundra lake on the North Slope of Alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (DOM). A shift in community composition, measured by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes, occurred at 4°C in near-surface waters beneath seasonal ice and snow cover in spring. This shift was associated with an annual peak in bacterial productivity ([14C]leucine incorporation) driven by the large influx of labile terrestrial DOM associated with snow meltwater. A second shift occurred after the flux of terrestrial DOM had ended in early summer as ice left the lake and as the phytoplankton community developed. Bacterioplankton communities were composed of persistent populations present throughout the year and transient populations that appeared and disappeared. Most of the transient populations could be divided into those that were advected into the lake with terrestrial DOM in spring and those that grew up from low concentrations during the development of the phytoplankton community in early summer. Sequencing of DNA in DGGE bands demonstrated that most bands represented single ribotypes and that matching bands from different samples represented identical ribotypes. Bacteria were identified as members of globally distributed freshwater phylogenetic clusters within the α- and β-Proteobacteria, the Cytophaga-Flavobacteria-Bacteroides group, and the Actinobacteria. Text Arctic north slope Phytoplankton Tundra Alaska PubMed Central (PMC) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Applied and Environmental Microbiology 69 4 2253 2268 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Crump, Byron C. Kling, George W. Bahr, Michele Hobbie, John E. Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
topic_facet |
Microbial Ecology |
description |
Seasonal shifts in bacterioplankton community composition in Toolik Lake, a tundra lake on the North Slope of Alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (DOM). A shift in community composition, measured by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes, occurred at 4°C in near-surface waters beneath seasonal ice and snow cover in spring. This shift was associated with an annual peak in bacterial productivity ([14C]leucine incorporation) driven by the large influx of labile terrestrial DOM associated with snow meltwater. A second shift occurred after the flux of terrestrial DOM had ended in early summer as ice left the lake and as the phytoplankton community developed. Bacterioplankton communities were composed of persistent populations present throughout the year and transient populations that appeared and disappeared. Most of the transient populations could be divided into those that were advected into the lake with terrestrial DOM in spring and those that grew up from low concentrations during the development of the phytoplankton community in early summer. Sequencing of DNA in DGGE bands demonstrated that most bands represented single ribotypes and that matching bands from different samples represented identical ribotypes. Bacteria were identified as members of globally distributed freshwater phylogenetic clusters within the α- and β-Proteobacteria, the Cytophaga-Flavobacteria-Bacteroides group, and the Actinobacteria. |
format |
Text |
author |
Crump, Byron C. Kling, George W. Bahr, Michele Hobbie, John E. |
author_facet |
Crump, Byron C. Kling, George W. Bahr, Michele Hobbie, John E. |
author_sort |
Crump, Byron C. |
title |
Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
title_short |
Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
title_full |
Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
title_fullStr |
Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
title_full_unstemmed |
Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source |
title_sort |
bacterioplankton community shifts in an arctic lake correlate with seasonal changes in organic matter source |
publisher |
American Society for Microbiology |
publishDate |
2003 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154827 http://www.ncbi.nlm.nih.gov/pubmed/12676708 https://doi.org/10.1128/AEM.69.4.2253-2268.2003 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake |
geographic_facet |
Arctic Arctic Lake |
genre |
Arctic north slope Phytoplankton Tundra Alaska |
genre_facet |
Arctic north slope Phytoplankton Tundra Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154827 http://www.ncbi.nlm.nih.gov/pubmed/12676708 http://dx.doi.org/10.1128/AEM.69.4.2253-2268.2003 |
op_rights |
Copyright © 2003, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.69.4.2253-2268.2003 |
container_title |
Applied and Environmental Microbiology |
container_volume |
69 |
container_issue |
4 |
container_start_page |
2253 |
op_container_end_page |
2268 |
_version_ |
1766340207741763584 |