Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characteriza...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
2003
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ftpubmed:oai:pubmedcentral.nih.gov:150077 2023-05-15T13:55:55+02:00 Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. 2003-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150077 http://www.ncbi.nlm.nih.gov/pubmed/12620829 https://doi.org/10.1128/AEM.69.3.1457-1464.2003 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150077 http://www.ncbi.nlm.nih.gov/pubmed/12620829 http://dx.doi.org/10.1128/AEM.69.3.1457-1464.2003 Copyright © 2003, American Society for Microbiology Environmental Microbiology and Biodegradation Text 2003 ftpubmed https://doi.org/10.1128/AEM.69.3.1457-1464.2003 2013-08-29T11:46:40Z Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45°C. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: α-, β-, and γ-Proteobacteria; the Flexibacter-Cytophaga-Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia. This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Applied and Environmental Microbiology 69 3 1457 1464 |
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English |
topic |
Environmental Microbiology and Biodegradation |
spellingShingle |
Environmental Microbiology and Biodegradation Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
topic_facet |
Environmental Microbiology and Biodegradation |
description |
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45°C. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: α-, β-, and γ-Proteobacteria; the Flexibacter-Cytophaga-Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia. This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. |
format |
Text |
author |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
author_facet |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
author_sort |
Wery, Nathalie |
title |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_short |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_full |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_fullStr |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_full_unstemmed |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_sort |
use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
publisher |
American Society for Microbiology |
publishDate |
2003 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150077 http://www.ncbi.nlm.nih.gov/pubmed/12620829 https://doi.org/10.1128/AEM.69.3.1457-1464.2003 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150077 http://www.ncbi.nlm.nih.gov/pubmed/12620829 http://dx.doi.org/10.1128/AEM.69.3.1457-1464.2003 |
op_rights |
Copyright © 2003, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.69.3.1457-1464.2003 |
container_title |
Applied and Environmental Microbiology |
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69 |
container_issue |
3 |
container_start_page |
1457 |
op_container_end_page |
1464 |
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1766262909554393088 |