RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W
The Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W has been used as a model system to identify genes that are required for growth at low temperature. Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4° but normal at 22°. In o...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449786 http://www.ncbi.nlm.nih.gov/pubmed/15956672 https://doi.org/10.1534/genetics.104.038943 |
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ftpubmed:oai:pubmedcentral.nih.gov:1449786 2023-05-15T14:05:24+02:00 RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W Regha, K. Satapathy, Ajit K. Ray, Malay K. 2005-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449786 http://www.ncbi.nlm.nih.gov/pubmed/15956672 https://doi.org/10.1534/genetics.104.038943 en eng Genetics Society of America http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449786 http://www.ncbi.nlm.nih.gov/pubmed/15956672 http://dx.doi.org/10.1534/genetics.104.038943 Copyright © 2005, Genetics Society of America Investigations Text 2005 ftpubmed https://doi.org/10.1534/genetics.104.038943 2013-08-31T00:58:24Z The Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W has been used as a model system to identify genes that are required for growth at low temperature. Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4° but normal at 22°. In one such cold-sensitive mutant (CS1), the transposon-disrupted gene was identified to be a homolog of the recD gene of several bacteria. Trans-complementation and freshly targeted gene disruption studies reconfirmed that the inactivation of the recD gene leads to a cold-sensitive phenotype. We cloned, sequenced, and analyzed ∼11.2 kbp of DNA from recD and its flanking region from the bacterium. recD was the last gene of a putative recCBD operon. The RecD ORF was 694 amino acids long and 40% identical (52% similar) to the Escherichia coli protein, and it could complement the E. coli recD mutation. The recD gene of E. coli, however, could not complement the cold-sensitive phenotype of the CS1 mutant. Interestingly, the CS1 strain showed greater sensitivity toward the DNA-damaging agents, mitomycin C and UV. The inactivation of recD in P. syringae also led to cell death and accumulation of DNA fragments of ∼25–30 kbp in size at low temperature (4°). We propose that during growth at a very low temperature the Antarctic P. syringae is subjected to DNA damage, which requires direct participation of a unique RecD function. Additional results suggest that a truncated recD encoding the N-terminal segment of (1–576) amino acids is sufficient to support growth of P. syringae at low temperature. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Genetics 170 4 1473 1484 |
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Investigations |
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Investigations Regha, K. Satapathy, Ajit K. Ray, Malay K. RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
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Investigations |
description |
The Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W has been used as a model system to identify genes that are required for growth at low temperature. Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4° but normal at 22°. In one such cold-sensitive mutant (CS1), the transposon-disrupted gene was identified to be a homolog of the recD gene of several bacteria. Trans-complementation and freshly targeted gene disruption studies reconfirmed that the inactivation of the recD gene leads to a cold-sensitive phenotype. We cloned, sequenced, and analyzed ∼11.2 kbp of DNA from recD and its flanking region from the bacterium. recD was the last gene of a putative recCBD operon. The RecD ORF was 694 amino acids long and 40% identical (52% similar) to the Escherichia coli protein, and it could complement the E. coli recD mutation. The recD gene of E. coli, however, could not complement the cold-sensitive phenotype of the CS1 mutant. Interestingly, the CS1 strain showed greater sensitivity toward the DNA-damaging agents, mitomycin C and UV. The inactivation of recD in P. syringae also led to cell death and accumulation of DNA fragments of ∼25–30 kbp in size at low temperature (4°). We propose that during growth at a very low temperature the Antarctic P. syringae is subjected to DNA damage, which requires direct participation of a unique RecD function. Additional results suggest that a truncated recD encoding the N-terminal segment of (1–576) amino acids is sufficient to support growth of P. syringae at low temperature. |
format |
Text |
author |
Regha, K. Satapathy, Ajit K. Ray, Malay K. |
author_facet |
Regha, K. Satapathy, Ajit K. Ray, Malay K. |
author_sort |
Regha, K. |
title |
RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
title_short |
RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
title_full |
RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
title_fullStr |
RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
title_full_unstemmed |
RecD Plays an Essential Function During Growth at Low Temperature in the Antarctic Bacterium Pseudomonas syringae Lz4W |
title_sort |
recd plays an essential function during growth at low temperature in the antarctic bacterium pseudomonas syringae lz4w |
publisher |
Genetics Society of America |
publishDate |
2005 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449786 http://www.ncbi.nlm.nih.gov/pubmed/15956672 https://doi.org/10.1534/genetics.104.038943 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449786 http://www.ncbi.nlm.nih.gov/pubmed/15956672 http://dx.doi.org/10.1534/genetics.104.038943 |
op_rights |
Copyright © 2005, Genetics Society of America |
op_doi |
https://doi.org/10.1534/genetics.104.038943 |
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Genetics |
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170 |
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4 |
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1473 |
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1484 |
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