Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments
As part of an ongoing effort to catalog spore-forming bacterial populations in environments conducive to interplanetary transfer by natural impacts or by human spaceflight activities, spores of Bacillus spp. were isolated and characterized from the interior of near-subsurface granite rock collected...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology
2006
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449054 http://www.ncbi.nlm.nih.gov/pubmed/16597992 https://doi.org/10.1128/AEM.72.4.2856-2863.2006 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:1449054 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:1449054 2023-05-15T14:05:24+02:00 Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments Fajardo-Cavazos, Patricia Nicholson, Wayne 2006-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449054 http://www.ncbi.nlm.nih.gov/pubmed/16597992 https://doi.org/10.1128/AEM.72.4.2856-2863.2006 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449054 http://www.ncbi.nlm.nih.gov/pubmed/16597992 http://dx.doi.org/10.1128/AEM.72.4.2856-2863.2006 Copyright © 2006, American Society for Microbiology Geomicrobiology Text 2006 ftpubmed https://doi.org/10.1128/AEM.72.4.2856-2863.2006 2013-08-31T00:55:53Z As part of an ongoing effort to catalog spore-forming bacterial populations in environments conducive to interplanetary transfer by natural impacts or by human spaceflight activities, spores of Bacillus spp. were isolated and characterized from the interior of near-subsurface granite rock collected from the Santa Catalina Mountains, AZ. Granite was found to contain ∼500 cultivable Bacillus spores and ∼104 total cultivable bacteria per gram. Many of the Bacillus isolates produced a previously unreported diffusible blue fluorescent compound. Two strains of eight tested exhibited increased spore UV resistance relative to a standard Bacillus subtilis UV biodosimetry strain. Fifty-six isolates were identified by repetitive extragenic palindromic PCR (rep-PCR) and 16S rRNA gene analysis as most closely related to B. megaterium (15 isolates), B. simplex (23 isolates), B. drentensis (6 isolates), B. niacini (7 isolates), and, likely, a new species related to B. barbaricus (5 isolates). Granite isolates were very closely related to a limited number of Bacillus spp. previously found to inhabit (i) globally distributed endolithic sites such as biodeteriorated murals, stone tombs, underground caverns, and rock concretions and (ii) extreme environments such as Antarctic soils, deep sea floor sediments, and spacecraft assembly facilities. Thus, it appears that the occurrence of Bacillus spp. in endolithic or extreme environments is not accidental but that these environments create unique niches excluding most Bacillus spp. but to which a limited number of Bacillus spp. are specifically adapted. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Catalina ENVELOPE(-59.633,-59.633,-62.333,-62.333) Applied and Environmental Microbiology 72 4 2856 2863 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Geomicrobiology |
spellingShingle |
Geomicrobiology Fajardo-Cavazos, Patricia Nicholson, Wayne Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
topic_facet |
Geomicrobiology |
description |
As part of an ongoing effort to catalog spore-forming bacterial populations in environments conducive to interplanetary transfer by natural impacts or by human spaceflight activities, spores of Bacillus spp. were isolated and characterized from the interior of near-subsurface granite rock collected from the Santa Catalina Mountains, AZ. Granite was found to contain ∼500 cultivable Bacillus spores and ∼104 total cultivable bacteria per gram. Many of the Bacillus isolates produced a previously unreported diffusible blue fluorescent compound. Two strains of eight tested exhibited increased spore UV resistance relative to a standard Bacillus subtilis UV biodosimetry strain. Fifty-six isolates were identified by repetitive extragenic palindromic PCR (rep-PCR) and 16S rRNA gene analysis as most closely related to B. megaterium (15 isolates), B. simplex (23 isolates), B. drentensis (6 isolates), B. niacini (7 isolates), and, likely, a new species related to B. barbaricus (5 isolates). Granite isolates were very closely related to a limited number of Bacillus spp. previously found to inhabit (i) globally distributed endolithic sites such as biodeteriorated murals, stone tombs, underground caverns, and rock concretions and (ii) extreme environments such as Antarctic soils, deep sea floor sediments, and spacecraft assembly facilities. Thus, it appears that the occurrence of Bacillus spp. in endolithic or extreme environments is not accidental but that these environments create unique niches excluding most Bacillus spp. but to which a limited number of Bacillus spp. are specifically adapted. |
format |
Text |
author |
Fajardo-Cavazos, Patricia Nicholson, Wayne |
author_facet |
Fajardo-Cavazos, Patricia Nicholson, Wayne |
author_sort |
Fajardo-Cavazos, Patricia |
title |
Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
title_short |
Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
title_full |
Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
title_fullStr |
Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
title_full_unstemmed |
Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments |
title_sort |
bacillus endospores isolated from granite: close molecular relationships to globally distributed bacillus spp. from endolithic and extreme environments |
publisher |
American Society for Microbiology |
publishDate |
2006 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449054 http://www.ncbi.nlm.nih.gov/pubmed/16597992 https://doi.org/10.1128/AEM.72.4.2856-2863.2006 |
long_lat |
ENVELOPE(-59.633,-59.633,-62.333,-62.333) |
geographic |
Antarctic Catalina |
geographic_facet |
Antarctic Catalina |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449054 http://www.ncbi.nlm.nih.gov/pubmed/16597992 http://dx.doi.org/10.1128/AEM.72.4.2856-2863.2006 |
op_rights |
Copyright © 2006, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.72.4.2856-2863.2006 |
container_title |
Applied and Environmental Microbiology |
container_volume |
72 |
container_issue |
4 |
container_start_page |
2856 |
op_container_end_page |
2863 |
_version_ |
1766277246059806720 |