Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity.
A prepared polysaccharide from the cell wall of yeast, M-Glucan, has previously been demonstrated to have immunostimulatory effects in salmonids as observed by enhanced in vivo non-specific disease resistance in Atlantic salmon, Salmo salar L., and increased in vitro bactericidal activity of rainbow...
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ftpubmed:oai:pubmedcentral.nih.gov:1414938 2023-05-15T15:30:17+02:00 Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. Brattgjerd, S Evensen, O Lauve, A 1994-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1414938 http://www.ncbi.nlm.nih.gov/pubmed/7835949 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1414938 http://www.ncbi.nlm.nih.gov/pubmed/7835949 Research Article Text 1994 ftpubmed 2013-08-30T23:16:32Z A prepared polysaccharide from the cell wall of yeast, M-Glucan, has previously been demonstrated to have immunostimulatory effects in salmonids as observed by enhanced in vivo non-specific disease resistance in Atlantic salmon, Salmo salar L., and increased in vitro bactericidal activity of rainbow trout, Oncorhynchus mykiss (Walbaum), macrophages. In the present study M-Glucan was injected intraperitoneally into Atlantic salmon and the effect on core components in the non-specific part of the immune system was observed. The hydrogen peroxide (H2O2) production of isolated head kidney macrophages from glucan-injected fish was measured 3 and 6 weeks after M-Glucan treatment and was increased at both time-points upon phorbol myristate acetate-(PMA) triggering. Without PMA triggering the difference was only significant 3 weeks after glucan injection when compared to a control group injected with saline. In a phagocytic assay with macrophages and Vibrio salmonicida the initial uptake of bacteria was elevated at both 3 and 6 weeks after glucan treatment. There was no significant difference when uptake of another fish pathogenic bacteria, Renibacterium salmoninarum, was studied. Treatment of Atlantic salmon with M-Glucan also resulted in enhanced serum lysozyme activity in week 3 of the experimental period. The results indicate that M-Glucan elevates the activity of the non-specific part of the immune system and the use of M-Glucan as an immunostimulant is discussed. Text Atlantic salmon Salmo salar PubMed Central (PMC) |
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Research Article Brattgjerd, S Evensen, O Lauve, A Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
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Research Article |
description |
A prepared polysaccharide from the cell wall of yeast, M-Glucan, has previously been demonstrated to have immunostimulatory effects in salmonids as observed by enhanced in vivo non-specific disease resistance in Atlantic salmon, Salmo salar L., and increased in vitro bactericidal activity of rainbow trout, Oncorhynchus mykiss (Walbaum), macrophages. In the present study M-Glucan was injected intraperitoneally into Atlantic salmon and the effect on core components in the non-specific part of the immune system was observed. The hydrogen peroxide (H2O2) production of isolated head kidney macrophages from glucan-injected fish was measured 3 and 6 weeks after M-Glucan treatment and was increased at both time-points upon phorbol myristate acetate-(PMA) triggering. Without PMA triggering the difference was only significant 3 weeks after glucan injection when compared to a control group injected with saline. In a phagocytic assay with macrophages and Vibrio salmonicida the initial uptake of bacteria was elevated at both 3 and 6 weeks after glucan treatment. There was no significant difference when uptake of another fish pathogenic bacteria, Renibacterium salmoninarum, was studied. Treatment of Atlantic salmon with M-Glucan also resulted in enhanced serum lysozyme activity in week 3 of the experimental period. The results indicate that M-Glucan elevates the activity of the non-specific part of the immune system and the use of M-Glucan as an immunostimulant is discussed. |
format |
Text |
author |
Brattgjerd, S Evensen, O Lauve, A |
author_facet |
Brattgjerd, S Evensen, O Lauve, A |
author_sort |
Brattgjerd, S |
title |
Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
title_short |
Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
title_full |
Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
title_fullStr |
Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
title_full_unstemmed |
Effect of injected yeast glucan on the activity of macrophages in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
title_sort |
effect of injected yeast glucan on the activity of macrophages in atlantic salmon, salmo salar l., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. |
publishDate |
1994 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1414938 http://www.ncbi.nlm.nih.gov/pubmed/7835949 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1414938 http://www.ncbi.nlm.nih.gov/pubmed/7835949 |
_version_ |
1766360731552317440 |