Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica
Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimat...
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ftpubmed:oai:pubmedcentral.nih.gov:1388784 2023-05-15T13:41:05+02:00 Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica Tibbles, B. J. Harris, J. M. 1996-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388784 http://www.ncbi.nlm.nih.gov/pubmed/16535246 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388784 http://www.ncbi.nlm.nih.gov/pubmed/16535246 Research Article Text 1996 ftpubmed 2013-08-30T22:05:36Z Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimates of bacterial productivity in soils near to bird nests (344 (plusmn) 422 ng of C g [dry weight](sup-1) h(sup-1)) were higher than those for soils from beneath mosses (175 (plusmn) 90 ng of C g [dry weight](sup-1) h(sup-1); measured by TTI at 10(deg)C), these differences were not significant because of patchiness of bacterial activity (P > 0.05). TTI- and [(sup14)C]leucine ([(sup14)C]Leu)-derived estimates of bacterial production were similar when incubations of 3 h were used, although incubations as short as 1 h were sufficient for measurable uptake of radiolabel. Dual-label incorporation of [(sup3)H]thymidine ([(sup3)H]TdR) into DNA and [(sup14)C]Leu into protein indicated that TTI did not reflect bacterial production of in situ assemblages when incubations were longer than 3 h. Isotope dilution analysis indicated that dilution of the specific activity of exogenously supplied [(sup3)H]TdR by de novo synthesis of TdR precursor could be limited by additions of [(sup3)H]TdR at a concentration of 1 nmol per ca. 115 mg of soil. TTI exhibited a psychrotrophic response to variation in temperature, with a temperature optimum of ca. 15(deg)C and a Q(inf10) value for 0 to 10(deg)C of 2.41. Text Antarc* Antarctic Antarctica Dronning Maud Land PubMed Central (PMC) Antarctic The Antarctic Dronning Maud Land Robertskollen ENVELOPE(-3.217,-3.217,-71.450,-71.450) |
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Research Article Tibbles, B. J. Harris, J. M. Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
topic_facet |
Research Article |
description |
Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimates of bacterial productivity in soils near to bird nests (344 (plusmn) 422 ng of C g [dry weight](sup-1) h(sup-1)) were higher than those for soils from beneath mosses (175 (plusmn) 90 ng of C g [dry weight](sup-1) h(sup-1); measured by TTI at 10(deg)C), these differences were not significant because of patchiness of bacterial activity (P > 0.05). TTI- and [(sup14)C]leucine ([(sup14)C]Leu)-derived estimates of bacterial production were similar when incubations of 3 h were used, although incubations as short as 1 h were sufficient for measurable uptake of radiolabel. Dual-label incorporation of [(sup3)H]thymidine ([(sup3)H]TdR) into DNA and [(sup14)C]Leu into protein indicated that TTI did not reflect bacterial production of in situ assemblages when incubations were longer than 3 h. Isotope dilution analysis indicated that dilution of the specific activity of exogenously supplied [(sup3)H]TdR by de novo synthesis of TdR precursor could be limited by additions of [(sup3)H]TdR at a concentration of 1 nmol per ca. 115 mg of soil. TTI exhibited a psychrotrophic response to variation in temperature, with a temperature optimum of ca. 15(deg)C and a Q(inf10) value for 0 to 10(deg)C of 2.41. |
format |
Text |
author |
Tibbles, B. J. Harris, J. M. |
author_facet |
Tibbles, B. J. Harris, J. M. |
author_sort |
Tibbles, B. J. |
title |
Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
title_short |
Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
title_full |
Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
title_fullStr |
Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
title_full_unstemmed |
Use of Radiolabelled Thymidine and Leucine To Estimate Bacterial Production in Soils from Continental Antarctica |
title_sort |
use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
publishDate |
1996 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388784 http://www.ncbi.nlm.nih.gov/pubmed/16535246 |
long_lat |
ENVELOPE(-3.217,-3.217,-71.450,-71.450) |
geographic |
Antarctic The Antarctic Dronning Maud Land Robertskollen |
geographic_facet |
Antarctic The Antarctic Dronning Maud Land Robertskollen |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388784 http://www.ncbi.nlm.nih.gov/pubmed/16535246 |
_version_ |
1766145510598508544 |