Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar)
Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common cha...
Published in: | Antimicrobial Agents and Chemotherapy |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology
2006
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1346769 http://www.ncbi.nlm.nih.gov/pubmed/16377685 https://doi.org/10.1128/AAC.50.1.185-195.2006 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:1346769 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:1346769 2023-05-15T15:31:44+02:00 Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) Chang, Chin-I Zhang, Yong-An Zou, Jun Nie, Pin Secombes, Christopher J. 2006-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1346769 http://www.ncbi.nlm.nih.gov/pubmed/16377685 https://doi.org/10.1128/AAC.50.1.185-195.2006 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1346769 http://www.ncbi.nlm.nih.gov/pubmed/16377685 http://dx.doi.org/10.1128/AAC.50.1.185-195.2006 Copyright © 2006, American Society for Microbiology Mechanisms of Resistance Text 2006 ftpubmed https://doi.org/10.1128/AAC.50.1.185-195.2006 2013-08-30T20:06:46Z Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon IV. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates. Text Atlantic salmon Salmo salar PubMed Central (PMC) Antimicrobial Agents and Chemotherapy 50 1 185 195 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Mechanisms of Resistance |
spellingShingle |
Mechanisms of Resistance Chang, Chin-I Zhang, Yong-An Zou, Jun Nie, Pin Secombes, Christopher J. Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
topic_facet |
Mechanisms of Resistance |
description |
Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon IV. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates. |
format |
Text |
author |
Chang, Chin-I Zhang, Yong-An Zou, Jun Nie, Pin Secombes, Christopher J. |
author_facet |
Chang, Chin-I Zhang, Yong-An Zou, Jun Nie, Pin Secombes, Christopher J. |
author_sort |
Chang, Chin-I |
title |
Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
title_short |
Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
title_full |
Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
title_fullStr |
Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Two Cathelicidin Genes Are Present in both Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar) |
title_sort |
two cathelicidin genes are present in both rainbow trout (oncorhynchus mykiss) and atlantic salmon (salmo salar) |
publisher |
American Society for Microbiology |
publishDate |
2006 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1346769 http://www.ncbi.nlm.nih.gov/pubmed/16377685 https://doi.org/10.1128/AAC.50.1.185-195.2006 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1346769 http://www.ncbi.nlm.nih.gov/pubmed/16377685 http://dx.doi.org/10.1128/AAC.50.1.185-195.2006 |
op_rights |
Copyright © 2006, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AAC.50.1.185-195.2006 |
container_title |
Antimicrobial Agents and Chemotherapy |
container_volume |
50 |
container_issue |
1 |
container_start_page |
185 |
op_container_end_page |
195 |
_version_ |
1766362259805700096 |