Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea†
Sequence analysis of environmental DNA promises to provide new insights into the ecology and biogeochemistry of uncultured marine microbes. In this study we used the Sargasso Sea Whole Genome Sequence (WGS) data set to search for hydrolases used by Cytophaga-like bacteria to degrade biopolymers such...
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ftpubmed:oai:pubmedcentral.nih.gov:1317373 2023-05-15T14:54:44+02:00 Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† Cottrell, Matthew T. Yu, Liying Kirchman, David L. 2005-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317373 http://www.ncbi.nlm.nih.gov/pubmed/16332841 https://doi.org/10.1128/AEM.71.12.8506-8513.2005 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317373 http://www.ncbi.nlm.nih.gov/pubmed/16332841 http://dx.doi.org/10.1128/AEM.71.12.8506-8513.2005 Copyright © 2005, American Society for Microbiology Environmental Microbiology Text 2005 ftpubmed https://doi.org/10.1128/AEM.71.12.8506-8513.2005 2013-08-30T18:45:43Z Sequence analysis of environmental DNA promises to provide new insights into the ecology and biogeochemistry of uncultured marine microbes. In this study we used the Sargasso Sea Whole Genome Sequence (WGS) data set to search for hydrolases used by Cytophaga-like bacteria to degrade biopolymers such as polysaccharides and proteins. Analysis of the Sargasso WGS data for contigs bearing both the 16S rRNA genes of Cytophaga-like bacteria and hydrolase genes revealed a cellulase gene (celM) most similar to the gene found in Cytophaga hutchinsonii. A BLAST search of the entire Sargasso Sea WGS data set indicated that celM was the most abundant cellulase-like gene in the Sargasso Sea. However, the similarity between CelM-like cellulases and peptidases belonging to metalloprotease family M42 led us to question whether CelM is involved in the degradation of polysaccharides or proteins. PCR primers were designed for the celM genes in the Sargasso Sea WGS data set and used to identify celM in a fosmid library constructed with prokaryotic DNA from the western Arctic Ocean. Expression analysis of the Cytophaga-like Arctic CelM, which is 63% identical and 77% similar to CelM in C. hutchinsonii, indicated that there was peptidase activity, whereas cellulase activity was not detected. Our analysis suggests that the celM gene plays a role in the degradation of protein by Cytophaga-like bacteria. The abundance of peptidase genes in the Cytophaga-like fosmid clone provides further evidence for the importance of Cytophaga-like bacteria in the degradation of protein in high-molecular-weight dissolved organic matter. Text Arctic Arctic Ocean PubMed Central (PMC) Arctic Arctic Ocean Applied and Environmental Microbiology 71 12 8506 8513 |
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op_collection_id |
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language |
English |
topic |
Environmental Microbiology |
spellingShingle |
Environmental Microbiology Cottrell, Matthew T. Yu, Liying Kirchman, David L. Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
topic_facet |
Environmental Microbiology |
description |
Sequence analysis of environmental DNA promises to provide new insights into the ecology and biogeochemistry of uncultured marine microbes. In this study we used the Sargasso Sea Whole Genome Sequence (WGS) data set to search for hydrolases used by Cytophaga-like bacteria to degrade biopolymers such as polysaccharides and proteins. Analysis of the Sargasso WGS data for contigs bearing both the 16S rRNA genes of Cytophaga-like bacteria and hydrolase genes revealed a cellulase gene (celM) most similar to the gene found in Cytophaga hutchinsonii. A BLAST search of the entire Sargasso Sea WGS data set indicated that celM was the most abundant cellulase-like gene in the Sargasso Sea. However, the similarity between CelM-like cellulases and peptidases belonging to metalloprotease family M42 led us to question whether CelM is involved in the degradation of polysaccharides or proteins. PCR primers were designed for the celM genes in the Sargasso Sea WGS data set and used to identify celM in a fosmid library constructed with prokaryotic DNA from the western Arctic Ocean. Expression analysis of the Cytophaga-like Arctic CelM, which is 63% identical and 77% similar to CelM in C. hutchinsonii, indicated that there was peptidase activity, whereas cellulase activity was not detected. Our analysis suggests that the celM gene plays a role in the degradation of protein by Cytophaga-like bacteria. The abundance of peptidase genes in the Cytophaga-like fosmid clone provides further evidence for the importance of Cytophaga-like bacteria in the degradation of protein in high-molecular-weight dissolved organic matter. |
format |
Text |
author |
Cottrell, Matthew T. Yu, Liying Kirchman, David L. |
author_facet |
Cottrell, Matthew T. Yu, Liying Kirchman, David L. |
author_sort |
Cottrell, Matthew T. |
title |
Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
title_short |
Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
title_full |
Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
title_fullStr |
Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
title_full_unstemmed |
Sequence and Expression Analyses of Cytophaga-Like Hydrolases in a Western Arctic Metagenomic Library and the Sargasso Sea† |
title_sort |
sequence and expression analyses of cytophaga-like hydrolases in a western arctic metagenomic library and the sargasso sea† |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317373 http://www.ncbi.nlm.nih.gov/pubmed/16332841 https://doi.org/10.1128/AEM.71.12.8506-8513.2005 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317373 http://www.ncbi.nlm.nih.gov/pubmed/16332841 http://dx.doi.org/10.1128/AEM.71.12.8506-8513.2005 |
op_rights |
Copyright © 2005, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.71.12.8506-8513.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
12 |
container_start_page |
8506 |
op_container_end_page |
8513 |
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1766326501033115648 |