Chitinase Genes in Lake Sediments of Ardley Island, Antarctica
A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers...
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ftpubmed:oai:pubmedcentral.nih.gov:1317360 2023-05-15T13:44:10+02:00 Chitinase Genes in Lake Sediments of Ardley Island, Antarctica Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping 2005-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317360 http://www.ncbi.nlm.nih.gov/pubmed/16332766 https://doi.org/10.1128/AEM.71.12.7904-7909.2005 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317360 http://www.ncbi.nlm.nih.gov/pubmed/16332766 http://dx.doi.org/10.1128/AEM.71.12.7904-7909.2005 Copyright © 2005, American Society for Microbiology Microbial Ecology Text 2005 ftpubmed https://doi.org/10.1128/AEM.71.12.7904-7909.2005 2013-08-30T18:45:43Z A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers targeting conserved sequences of the catalytic domains of family 18 subgroup A chitinases detected group A chitinases from a wide taxonomic range of bacteria. Using quantitative competitive PCR (QC-PCR), chitinase gene copies in each 1-cm section of the whole sediment column were quantified. QC-PCR determination of the chitinase gene copies indicated significant correlation with phosphorus and total organic carbon concentration, suggesting a historical connection between chitinase gene copies and the amount of penguin guano input into the lake sediment. Most of the chitinase genes cloned from the historic sediment core were novel. Analysis of the chitinase gene diversity in selected sediment layers and in the fresh penguin deposits indicated frequent shifts in the chitinolytic bacterial community over time. Sequence analysis of the 16S rRNA genes of chitinolytic bacteria isolated from the lake sediment revealed that the isolates belonged to Janthinobacterium species, Stenotrophomonas species of γ-Proteobacteria, Cytophaga species of the Cytophaga-Flexibacter-Bacteroides group, and Streptomyces and Norcardiopsis species of Actinobacteria. Chitinase gene fragments were cloned and sequenced from these cultivated chitinolytic bacteria. The phylogeny of the chitinase genes obtained from the isolates did not correspond well to that of the isolates, suggesting acquisition via horizontal gene transfer. Text Antarc* Antarctica Ardley Island PubMed Central (PMC) Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Ardley ENVELOPE(-58.953,-58.953,-62.201,-62.201) Ardley Island ENVELOPE(-58.933,-58.933,-62.213,-62.213) Applied and Environmental Microbiology 71 12 7904 7909 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
topic_facet |
Microbial Ecology |
description |
A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers targeting conserved sequences of the catalytic domains of family 18 subgroup A chitinases detected group A chitinases from a wide taxonomic range of bacteria. Using quantitative competitive PCR (QC-PCR), chitinase gene copies in each 1-cm section of the whole sediment column were quantified. QC-PCR determination of the chitinase gene copies indicated significant correlation with phosphorus and total organic carbon concentration, suggesting a historical connection between chitinase gene copies and the amount of penguin guano input into the lake sediment. Most of the chitinase genes cloned from the historic sediment core were novel. Analysis of the chitinase gene diversity in selected sediment layers and in the fresh penguin deposits indicated frequent shifts in the chitinolytic bacterial community over time. Sequence analysis of the 16S rRNA genes of chitinolytic bacteria isolated from the lake sediment revealed that the isolates belonged to Janthinobacterium species, Stenotrophomonas species of γ-Proteobacteria, Cytophaga species of the Cytophaga-Flexibacter-Bacteroides group, and Streptomyces and Norcardiopsis species of Actinobacteria. Chitinase gene fragments were cloned and sequenced from these cultivated chitinolytic bacteria. The phylogeny of the chitinase genes obtained from the isolates did not correspond well to that of the isolates, suggesting acquisition via horizontal gene transfer. |
format |
Text |
author |
Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping |
author_facet |
Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping |
author_sort |
Xiao, Xiang |
title |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_short |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_full |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_fullStr |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_full_unstemmed |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_sort |
chitinase genes in lake sediments of ardley island, antarctica |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317360 http://www.ncbi.nlm.nih.gov/pubmed/16332766 https://doi.org/10.1128/AEM.71.12.7904-7909.2005 |
long_lat |
ENVELOPE(141.604,141.604,-66.775,-66.775) ENVELOPE(-58.953,-58.953,-62.201,-62.201) ENVELOPE(-58.933,-58.933,-62.213,-62.213) |
geographic |
Guano Ardley Ardley Island |
geographic_facet |
Guano Ardley Ardley Island |
genre |
Antarc* Antarctica Ardley Island |
genre_facet |
Antarc* Antarctica Ardley Island |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317360 http://www.ncbi.nlm.nih.gov/pubmed/16332766 http://dx.doi.org/10.1128/AEM.71.12.7904-7909.2005 |
op_rights |
Copyright © 2005, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.71.12.7904-7909.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
12 |
container_start_page |
7904 |
op_container_end_page |
7909 |
_version_ |
1766198304314490880 |