Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine
dl-2-amino-Δ2-thiazolin-4-carbonic acid (dl-ATC) is a substrate for cysteine synthesis in some bacteria, and this bioconversion has been utilized for cysteine production in industry. We cloned a DNA fragment containing the genes involved in the conversion of l-ATC to l-cysteine from Pseudomonas sp....
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ftpubmed:oai:pubmedcentral.nih.gov:127550 2023-05-15T15:52:35+02:00 Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine Shiba, Toshikazu Takeda, Kohji Yajima, Misako Tadano, Makoto 2002-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127550 http://www.ncbi.nlm.nih.gov/pubmed/11976087 https://doi.org/10.1128/AEM.68.5.2179-2187.2002 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127550 http://www.ncbi.nlm.nih.gov/pubmed/11976087 http://dx.doi.org/10.1128/AEM.68.5.2179-2187.2002 Copyright © 2002, American Society for Microbiology Genetics and Molecular Biology Text 2002 ftpubmed https://doi.org/10.1128/AEM.68.5.2179-2187.2002 2013-08-29T10:58:24Z dl-2-amino-Δ2-thiazolin-4-carbonic acid (dl-ATC) is a substrate for cysteine synthesis in some bacteria, and this bioconversion has been utilized for cysteine production in industry. We cloned a DNA fragment containing the genes involved in the conversion of l-ATC to l-cysteine from Pseudomonas sp. strain BS. The introduction of this DNA fragment into Escherichia coli cells enabled them to convert l-ATC to cysteine via N-carbamyl-l-cysteine (l-NCC) as an intermediate. The smallest recombinant plasmid, designated pTK10, contained a 2.6-kb insert DNA fragment that has l-cysteine synthetic activity. The nucleotide sequence of the insert DNA revealed that two open reading frames (ORFs) encoding proteins with molecular masses of 19.5 and 44.7 kDa were involved in the l-cysteine synthesis from dl-ATC. These ORFs were designated atcB and atcC, respectively, and their gene products were identified by overproduction of proteins encoded in each ORF and by the maxicell method. The functions of these gene products were examined using extracts of E. coli cells carrying deletion derivatives of pTK10. The results indicate that atcB and atcC are involved in the conversion of l-ATC to l-NCC and the conversion of l-NCC to cysteine, respectively. atcB was first identified as a gene encoding an enzyme that catalyzes thiazolin ring opening. AtcC is highly homologous with l-N-carbamoylases. Since both enzymes can only catalyze the l-specific conversion from l-ATC to l-NCC or l-NCC to l-cysteine, it is thought that atcB and atcC encode l-ATC hydrolase and N-carbamyl-l-cysteine amidohydrolase, respectively. Text Carbonic acid PubMed Central (PMC) Applied and Environmental Microbiology 68 5 2179 2187 |
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Genetics and Molecular Biology Shiba, Toshikazu Takeda, Kohji Yajima, Misako Tadano, Makoto Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
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Genetics and Molecular Biology |
description |
dl-2-amino-Δ2-thiazolin-4-carbonic acid (dl-ATC) is a substrate for cysteine synthesis in some bacteria, and this bioconversion has been utilized for cysteine production in industry. We cloned a DNA fragment containing the genes involved in the conversion of l-ATC to l-cysteine from Pseudomonas sp. strain BS. The introduction of this DNA fragment into Escherichia coli cells enabled them to convert l-ATC to cysteine via N-carbamyl-l-cysteine (l-NCC) as an intermediate. The smallest recombinant plasmid, designated pTK10, contained a 2.6-kb insert DNA fragment that has l-cysteine synthetic activity. The nucleotide sequence of the insert DNA revealed that two open reading frames (ORFs) encoding proteins with molecular masses of 19.5 and 44.7 kDa were involved in the l-cysteine synthesis from dl-ATC. These ORFs were designated atcB and atcC, respectively, and their gene products were identified by overproduction of proteins encoded in each ORF and by the maxicell method. The functions of these gene products were examined using extracts of E. coli cells carrying deletion derivatives of pTK10. The results indicate that atcB and atcC are involved in the conversion of l-ATC to l-NCC and the conversion of l-NCC to cysteine, respectively. atcB was first identified as a gene encoding an enzyme that catalyzes thiazolin ring opening. AtcC is highly homologous with l-N-carbamoylases. Since both enzymes can only catalyze the l-specific conversion from l-ATC to l-NCC or l-NCC to l-cysteine, it is thought that atcB and atcC encode l-ATC hydrolase and N-carbamyl-l-cysteine amidohydrolase, respectively. |
format |
Text |
author |
Shiba, Toshikazu Takeda, Kohji Yajima, Misako Tadano, Makoto |
author_facet |
Shiba, Toshikazu Takeda, Kohji Yajima, Misako Tadano, Makoto |
author_sort |
Shiba, Toshikazu |
title |
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
title_short |
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
title_full |
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
title_fullStr |
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
title_full_unstemmed |
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine |
title_sort |
genes from pseudomonas sp. strain bs involved in the conversion of l-2-amino-δ2-thiazolin-4-carbonic acid to l-cysteine |
publisher |
American Society for Microbiology |
publishDate |
2002 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127550 http://www.ncbi.nlm.nih.gov/pubmed/11976087 https://doi.org/10.1128/AEM.68.5.2179-2187.2002 |
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Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127550 http://www.ncbi.nlm.nih.gov/pubmed/11976087 http://dx.doi.org/10.1128/AEM.68.5.2179-2187.2002 |
op_rights |
Copyright © 2002, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.68.5.2179-2187.2002 |
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Applied and Environmental Microbiology |
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68 |
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5 |
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2179 |
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2187 |
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1766387719347372032 |