Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature
Algal-virus-specific PCR primers were used to amplify DNA polymerase (pol) gene fragments from geographically isolated natural virus communities. Natural algal virus communities were obtained from coastal sites in the Pacific Ocean in British Columbia, Canada, and the Southern Ocean near the Antarct...
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ftpubmed:oai:pubmedcentral.nih.gov:123764 2023-05-15T13:48:49+02:00 Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature Short, Steven M. Suttle, Curtis A. 2002-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123764 http://www.ncbi.nlm.nih.gov/pubmed/11872479 https://doi.org/10.1128/AEM.68.3.1290-1296.2002 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123764 http://www.ncbi.nlm.nih.gov/pubmed/11872479 http://dx.doi.org/10.1128/AEM.68.3.1290-1296.2002 Copyright © 2002, American Society for Microbiology Microbial Ecology Text 2002 ftpubmed https://doi.org/10.1128/AEM.68.3.1290-1296.2002 2013-08-29T10:47:48Z Algal-virus-specific PCR primers were used to amplify DNA polymerase (pol) gene fragments from geographically isolated natural virus communities. Natural algal virus communities were obtained from coastal sites in the Pacific Ocean in British Columbia, Canada, and the Southern Ocean near the Antarctic peninsula. Genetic fingerprints of algal virus communities were generated using denaturing gradient gel electrophoresis (DGGE). Sequencing efforts recovered 33 sequences from the gradient gel. Of the 33 sequences examined, 25 encoded a conserved amino acid motif indicating that the sequences were pol gene fragments. Furthermore, the 25 pol sequences were related to pol gene fragments from known algal viruses. In addition, similar virus sequences (>98% sequence identity) were recovered from British Columbia and Antarctica. Results from this study demonstrate that DGGE with degenerate primers can be used to qualitatively fingerprint and assess genetic diversity in specific subsets of natural virus communities and that closely related viruses occur in distant geographic locations. DGGE is a powerful tool for genetically fingerprinting natural virus communities and may be used to examine how specific components of virus communities respond to experimental manipulations. Text Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean PubMed Central (PMC) Antarctic Antarctic Peninsula British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Canada Pacific Southern Ocean The Antarctic Applied and Environmental Microbiology 68 3 1290 1296 |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Short, Steven M. Suttle, Curtis A. Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
topic_facet |
Microbial Ecology |
description |
Algal-virus-specific PCR primers were used to amplify DNA polymerase (pol) gene fragments from geographically isolated natural virus communities. Natural algal virus communities were obtained from coastal sites in the Pacific Ocean in British Columbia, Canada, and the Southern Ocean near the Antarctic peninsula. Genetic fingerprints of algal virus communities were generated using denaturing gradient gel electrophoresis (DGGE). Sequencing efforts recovered 33 sequences from the gradient gel. Of the 33 sequences examined, 25 encoded a conserved amino acid motif indicating that the sequences were pol gene fragments. Furthermore, the 25 pol sequences were related to pol gene fragments from known algal viruses. In addition, similar virus sequences (>98% sequence identity) were recovered from British Columbia and Antarctica. Results from this study demonstrate that DGGE with degenerate primers can be used to qualitatively fingerprint and assess genetic diversity in specific subsets of natural virus communities and that closely related viruses occur in distant geographic locations. DGGE is a powerful tool for genetically fingerprinting natural virus communities and may be used to examine how specific components of virus communities respond to experimental manipulations. |
format |
Text |
author |
Short, Steven M. Suttle, Curtis A. |
author_facet |
Short, Steven M. Suttle, Curtis A. |
author_sort |
Short, Steven M. |
title |
Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
title_short |
Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
title_full |
Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
title_fullStr |
Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
title_full_unstemmed |
Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature |
title_sort |
sequence analysis of marine virus communities reveals that groups of related algal viruses are widely distributed in nature |
publisher |
American Society for Microbiology |
publishDate |
2002 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123764 http://www.ncbi.nlm.nih.gov/pubmed/11872479 https://doi.org/10.1128/AEM.68.3.1290-1296.2002 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) |
geographic |
Antarctic Antarctic Peninsula British Columbia Canada Pacific Southern Ocean The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula British Columbia Canada Pacific Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123764 http://www.ncbi.nlm.nih.gov/pubmed/11872479 http://dx.doi.org/10.1128/AEM.68.3.1290-1296.2002 |
op_rights |
Copyright © 2002, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.68.3.1290-1296.2002 |
container_title |
Applied and Environmental Microbiology |
container_volume |
68 |
container_issue |
3 |
container_start_page |
1290 |
op_container_end_page |
1296 |
_version_ |
1766249810122244096 |