Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish
To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that sh...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123123 http://www.ncbi.nlm.nih.gov/pubmed/12093923 https://doi.org/10.1073/pnas.152294399 |
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ftpubmed:oai:pubmedcentral.nih.gov:123123 2023-05-15T15:31:24+02:00 Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. M. Hebert, S. C. Harris, H. W. 2002-07-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123123 http://www.ncbi.nlm.nih.gov/pubmed/12093923 https://doi.org/10.1073/pnas.152294399 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123123 http://www.ncbi.nlm.nih.gov/pubmed/12093923 http://dx.doi.org/10.1073/pnas.152294399 Copyright © 2002, The National Academy of Sciences Biological Sciences Text 2002 ftpubmed https://doi.org/10.1073/pnas.152294399 2013-08-29T10:46:38Z To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of Ca2+, Mg2+, and Na+ ions at concentrations present in seawater and kidney tubules. Shark kidney is expressed in multiple shark osmoregulatory organs, including specific tubules of the kidney, rectal gland, stomach, intestine, olfactory lamellae, gill, and brain. Reverse transcriptase–PCR amplification using specific primers in two teleost fish, winter flounder (Pleuronectes americanus) and Atlantic salmon (Salmo salar), reveals a similar pattern of CaR tissue expression. Exposure of the lumen of winter flounder urinary bladder to the CaR agonists, Gd3+ and neomycin, reversibly inhibit volume transport, which is important for euryhaline teleost survival in seawater. Within 24–72 hr after transfer of freshwater-adapted Atlantic salmon to seawater, there are increases in their plasma Ca2+, Mg2+, and Na+ that likely serve as a signal for internal CaRs, i.e., brain, to sense alterations in salinity in the surrounding water. We conclude that CaRs act as salinity sensors in both teleost and elasmobranch fish. Their tissue expression patterns in fish provide insights into CaR functions in terrestrial animals including humans. Text Atlantic salmon Salmo salar Squalus acanthias PubMed Central (PMC) Proceedings of the National Academy of Sciences 99 14 9231 9236 |
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English |
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Biological Sciences |
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Biological Sciences Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. M. Hebert, S. C. Harris, H. W. Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
topic_facet |
Biological Sciences |
description |
To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of Ca2+, Mg2+, and Na+ ions at concentrations present in seawater and kidney tubules. Shark kidney is expressed in multiple shark osmoregulatory organs, including specific tubules of the kidney, rectal gland, stomach, intestine, olfactory lamellae, gill, and brain. Reverse transcriptase–PCR amplification using specific primers in two teleost fish, winter flounder (Pleuronectes americanus) and Atlantic salmon (Salmo salar), reveals a similar pattern of CaR tissue expression. Exposure of the lumen of winter flounder urinary bladder to the CaR agonists, Gd3+ and neomycin, reversibly inhibit volume transport, which is important for euryhaline teleost survival in seawater. Within 24–72 hr after transfer of freshwater-adapted Atlantic salmon to seawater, there are increases in their plasma Ca2+, Mg2+, and Na+ that likely serve as a signal for internal CaRs, i.e., brain, to sense alterations in salinity in the surrounding water. We conclude that CaRs act as salinity sensors in both teleost and elasmobranch fish. Their tissue expression patterns in fish provide insights into CaR functions in terrestrial animals including humans. |
format |
Text |
author |
Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. M. Hebert, S. C. Harris, H. W. |
author_facet |
Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. M. Hebert, S. C. Harris, H. W. |
author_sort |
Nearing, J. |
title |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_short |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_full |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_fullStr |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_full_unstemmed |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_sort |
polyvalent cation receptor proteins (cars) are salinity sensors in fish |
publisher |
National Academy of Sciences |
publishDate |
2002 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123123 http://www.ncbi.nlm.nih.gov/pubmed/12093923 https://doi.org/10.1073/pnas.152294399 |
genre |
Atlantic salmon Salmo salar Squalus acanthias |
genre_facet |
Atlantic salmon Salmo salar Squalus acanthias |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123123 http://www.ncbi.nlm.nih.gov/pubmed/12093923 http://dx.doi.org/10.1073/pnas.152294399 |
op_rights |
Copyright © 2002, The National Academy of Sciences |
op_doi |
https://doi.org/10.1073/pnas.152294399 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
99 |
container_issue |
14 |
container_start_page |
9231 |
op_container_end_page |
9236 |
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1766361896017985536 |