Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA.
Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking fe...
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ftpubmed:oai:pubmedcentral.nih.gov:1218178 2023-05-15T14:00:29+02:00 Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. Scudiero, R Carginale, V Riggio, M Capasso, C Capasso, A Kille, P di Prisco, G Parisi, E 1997-02-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218178 http://www.ncbi.nlm.nih.gov/pubmed/9078263 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218178 http://www.ncbi.nlm.nih.gov/pubmed/9078263 Research Article Text 1997 ftpubmed 2013-08-30T13:56:10Z Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking feature of the icefish is that their liver is devoid of metallothionein. These cysteine-rich heavy-metal-binding proteins are usually present in large amounts in a large variety of organisms, from bacteria to mammals. Despite the failure to detect appreciable levels of metallothionein in icefish liver, a cDNA encoding metallothionein was produced from total RNA by reverse transcriptase PCR. The icefish metallothionein showed high percentage identity with metallothionein from Trematomus bernachii, a red-blooded Antarctic fish in which a normal content of hepatic metallothionein was found. Steady-state mRNA levels were assessed in fish liver by high-stringency hybridization of the metallothionein probe with total RNA. The results showed that icefish livers retain large amounts of untranslated metallothionein mRNA. The stability of the icefish transcript might be correlated with the lack of specific motifs in the untranslated 3' ends of mRNA. Text Antarc* Antarctic Icefish PubMed Central (PMC) Antarctic |
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Research Article Scudiero, R Carginale, V Riggio, M Capasso, C Capasso, A Kille, P di Prisco, G Parisi, E Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
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Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking feature of the icefish is that their liver is devoid of metallothionein. These cysteine-rich heavy-metal-binding proteins are usually present in large amounts in a large variety of organisms, from bacteria to mammals. Despite the failure to detect appreciable levels of metallothionein in icefish liver, a cDNA encoding metallothionein was produced from total RNA by reverse transcriptase PCR. The icefish metallothionein showed high percentage identity with metallothionein from Trematomus bernachii, a red-blooded Antarctic fish in which a normal content of hepatic metallothionein was found. Steady-state mRNA levels were assessed in fish liver by high-stringency hybridization of the metallothionein probe with total RNA. The results showed that icefish livers retain large amounts of untranslated metallothionein mRNA. The stability of the icefish transcript might be correlated with the lack of specific motifs in the untranslated 3' ends of mRNA. |
format |
Text |
author |
Scudiero, R Carginale, V Riggio, M Capasso, C Capasso, A Kille, P di Prisco, G Parisi, E |
author_facet |
Scudiero, R Carginale, V Riggio, M Capasso, C Capasso, A Kille, P di Prisco, G Parisi, E |
author_sort |
Scudiero, R |
title |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
title_short |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
title_full |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
title_fullStr |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
title_full_unstemmed |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA. |
title_sort |
difference in hepatic metallothionein content in antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mrna. |
publishDate |
1997 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218178 http://www.ncbi.nlm.nih.gov/pubmed/9078263 |
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Antarctic |
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Antarctic |
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Antarc* Antarctic Icefish |
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Antarc* Antarctic Icefish |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218178 http://www.ncbi.nlm.nih.gov/pubmed/9078263 |
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1766269655561797632 |