Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha.
Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are cytokines produced primarily by monocytes and macrophages with regulatory effects in inflammation and multiple aspects of the immune response. As yet, no molecular data have been reported for IL-1beta and TNF-alpha of the b...
Main Authors: | , |
---|---|
Format: | Text |
Language: | English |
Published: |
2001
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189685 http://www.ncbi.nlm.nih.gov/pubmed/11768130 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:1189685 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:1189685 2023-05-15T15:41:26+02:00 Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. Denis, F Archambault, D 2001-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189685 http://www.ncbi.nlm.nih.gov/pubmed/11768130 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189685 http://www.ncbi.nlm.nih.gov/pubmed/11768130 Research Article Text 2001 ftpubmed 2013-08-30T12:32:58Z Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are cytokines produced primarily by monocytes and macrophages with regulatory effects in inflammation and multiple aspects of the immune response. As yet, no molecular data have been reported for IL-1beta and TNF-alpha of the beluga whale. In this study, we cloned and determined the entire cDNA sequence encoding beluga whale IL-1beta and TNF-alpha. The genetic relationship of the cytokine sequences was then analyzed with those from several mammalian species, including the human and the pig. The homology of beluga whale IL-1beta nucleic acid and deduced amino acid sequences with those from these mammalian species ranged from 74.6 to 86.0% and 62.7 to 77.1%, respectively, whereas that of TNF-alpha varied from 79.3 to 90.8% and 75.3 to 87.7%, respectively. Phylogenetic analyses based on deduced amino acid sequences showed that the beluga whale IL-1beta and TNF-alpha were most closely related to those of the ruminant species (cattle, sheep, and deer). The beluga whale IL-1beta- and TNF-alpha-encoding sequences were thereafter successfully expressed in Escherichia coli as fusion proteins by using procaryotic expression vectors. The fusion proteins were used to produce beluga whale IL-1beta- and TNF-alpha-specific rabbit antisera. Text Beluga Beluga whale Beluga* Delphinapterus leucas PubMed Central (PMC) |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Denis, F Archambault, D Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
topic_facet |
Research Article |
description |
Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are cytokines produced primarily by monocytes and macrophages with regulatory effects in inflammation and multiple aspects of the immune response. As yet, no molecular data have been reported for IL-1beta and TNF-alpha of the beluga whale. In this study, we cloned and determined the entire cDNA sequence encoding beluga whale IL-1beta and TNF-alpha. The genetic relationship of the cytokine sequences was then analyzed with those from several mammalian species, including the human and the pig. The homology of beluga whale IL-1beta nucleic acid and deduced amino acid sequences with those from these mammalian species ranged from 74.6 to 86.0% and 62.7 to 77.1%, respectively, whereas that of TNF-alpha varied from 79.3 to 90.8% and 75.3 to 87.7%, respectively. Phylogenetic analyses based on deduced amino acid sequences showed that the beluga whale IL-1beta and TNF-alpha were most closely related to those of the ruminant species (cattle, sheep, and deer). The beluga whale IL-1beta- and TNF-alpha-encoding sequences were thereafter successfully expressed in Escherichia coli as fusion proteins by using procaryotic expression vectors. The fusion proteins were used to produce beluga whale IL-1beta- and TNF-alpha-specific rabbit antisera. |
format |
Text |
author |
Denis, F Archambault, D |
author_facet |
Denis, F Archambault, D |
author_sort |
Denis, F |
title |
Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
title_short |
Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
title_full |
Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
title_fullStr |
Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
title_full_unstemmed |
Molecular cloning and characterization of beluga whale (Delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
title_sort |
molecular cloning and characterization of beluga whale (delphinapterus leucas) interleukin-1beta and tumor necrosis factor-alpha. |
publishDate |
2001 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189685 http://www.ncbi.nlm.nih.gov/pubmed/11768130 |
genre |
Beluga Beluga whale Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga whale Beluga* Delphinapterus leucas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1189685 http://www.ncbi.nlm.nih.gov/pubmed/11768130 |
_version_ |
1766374345255419904 |