The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate.
1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with d...
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ftpubmed:oai:pubmedcentral.nih.gov:1186641 2023-05-15T13:27:46+02:00 The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. Bell, M V Sargent, J R 1979-05-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1186641 http://www.ncbi.nlm.nih.gov/pubmed/39542 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1186641 http://www.ncbi.nlm.nih.gov/pubmed/39542 Research Article Text 1979 ftpubmed 2013-08-30T12:24:18Z 1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with detergent. 3. The final preparation (56% pure) had a specific activity of 341 mumol of ATP hydrolysed/h per mg of protein and a turnover number of 3560 min-1. The number of ouabain-binding sties equalled the number of sites phosphorylated by ATP. 4. Both sodium orthovanadate and ouabain inhibited the purified preparation more than the microsomal fraction, vanadate being more effective on an equimolar basis than ouabain. 5. Inhibition by orthovanadate was not enhanced at 28 mM-as compared with 1mM-MgCl2 and was not reversed by beta-adrenergic agonists (cf. Josephson & Cantley (1977) Biochemistry 16, 4572--4578). 6. Of various other metallic oxyanions tested only niobate proved an effective inhibitor of the enzyme although this anion was less effective than orthovanadate. 7. Orthovanadate partially inhibited phosphorylation of the enzyme by ATP in the presence of 28 mM-MgCl2. Text Anguilla anguilla PubMed Central (PMC) |
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Research Article Bell, M V Sargent, J R The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
topic_facet |
Research Article |
description |
1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with detergent. 3. The final preparation (56% pure) had a specific activity of 341 mumol of ATP hydrolysed/h per mg of protein and a turnover number of 3560 min-1. The number of ouabain-binding sties equalled the number of sites phosphorylated by ATP. 4. Both sodium orthovanadate and ouabain inhibited the purified preparation more than the microsomal fraction, vanadate being more effective on an equimolar basis than ouabain. 5. Inhibition by orthovanadate was not enhanced at 28 mM-as compared with 1mM-MgCl2 and was not reversed by beta-adrenergic agonists (cf. Josephson & Cantley (1977) Biochemistry 16, 4572--4578). 6. Of various other metallic oxyanions tested only niobate proved an effective inhibitor of the enzyme although this anion was less effective than orthovanadate. 7. Orthovanadate partially inhibited phosphorylation of the enzyme by ATP in the presence of 28 mM-MgCl2. |
format |
Text |
author |
Bell, M V Sargent, J R |
author_facet |
Bell, M V Sargent, J R |
author_sort |
Bell, M V |
title |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
title_short |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
title_full |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
title_fullStr |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
title_full_unstemmed |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate. |
title_sort |
partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of anguilla anguilla and its inhibition by orthovanadate. |
publishDate |
1979 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1186641 http://www.ncbi.nlm.nih.gov/pubmed/39542 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1186641 http://www.ncbi.nlm.nih.gov/pubmed/39542 |
_version_ |
1766400306571116544 |