Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparation...
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ftpubmed:oai:pubmedcentral.nih.gov:1179607 2023-05-15T18:26:41+02:00 Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers Boegman, R. J. Crumpton, M. J. 1970-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179607 http://www.ncbi.nlm.nih.gov/pubmed/5493858 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179607 http://www.ncbi.nlm.nih.gov/pubmed/5493858 Articles Text 1970 ftpubmed 2013-08-30T12:02:03Z Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose–myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60–100% of the antibody adsorbed by Sepharose–myoglobin, and 60–90% of the eluted protein was precipitable antibody. Text Sperm whale PubMed Central (PMC) |
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Articles Boegman, R. J. Crumpton, M. J. Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
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Articles |
description |
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose–myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60–100% of the antibody adsorbed by Sepharose–myoglobin, and 60–90% of the eluted protein was precipitable antibody. |
format |
Text |
author |
Boegman, R. J. Crumpton, M. J. |
author_facet |
Boegman, R. J. Crumpton, M. J. |
author_sort |
Boegman, R. J. |
title |
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
title_short |
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
title_full |
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
title_fullStr |
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
title_full_unstemmed |
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
title_sort |
comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers |
publishDate |
1970 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179607 http://www.ncbi.nlm.nih.gov/pubmed/5493858 |
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Sperm whale |
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Sperm whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1179607 http://www.ncbi.nlm.nih.gov/pubmed/5493858 |
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1766208652498173952 |