Two thermostable type II restriction endonucleases from Icelandic strains of the genus Thermus: Tsp4C I (ACN/GT), a novel type II restriction endonuclease, and Tsp8E I, an isoschizomer of the mesophilic enzyme Bgl I (GCCNNNN/NGGC).

• Main Authors: Welch, S G, Williams, R A
• Format: Text
• Language: English
• Published: 1995
• Subjects: Research Article; Lambda; Iceland
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1135772; http://www.ncbi.nlm.nih.gov/pubmed/7626025

Sixteen isolates of thermophilic bacteria from the genus Thermus, isolated from neutral and alkaline hot water springs in the southwest region of Iceland, were tested for the presence of restriction endonucleases. Extracts from five of the isolates showed evidence of the presence of restriction endo...