Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes†
The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermop...
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American Society for Microbiology
2005
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ftpubmed:oai:pubmedcentral.nih.gov:1087530 2023-05-15T16:49:33+02:00 Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. 2005-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087530 http://www.ncbi.nlm.nih.gov/pubmed/15870372 https://doi.org/10.1128/AEM.71.5.2771-2776.2005 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087530 http://www.ncbi.nlm.nih.gov/pubmed/15870372 http://dx.doi.org/10.1128/AEM.71.5.2771-2776.2005 Copyright © 2005, American Society for Microbiology Microbial Ecology Text 2005 ftpubmed https://doi.org/10.1128/AEM.71.5.2771-2776.2005 2013-08-30T08:40:48Z The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach. Text Iceland PubMed Central (PMC) Applied and Environmental Microbiology 71 5 2771 2776 |
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language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
topic_facet |
Microbial Ecology |
description |
The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach. |
format |
Text |
author |
Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. |
author_facet |
Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. |
author_sort |
Hobel, Cédric F. V. |
title |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
title_short |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
title_full |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
title_fullStr |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
title_full_unstemmed |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes† |
title_sort |
investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16s rrna and chitinase genes† |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087530 http://www.ncbi.nlm.nih.gov/pubmed/15870372 https://doi.org/10.1128/AEM.71.5.2771-2776.2005 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087530 http://www.ncbi.nlm.nih.gov/pubmed/15870372 http://dx.doi.org/10.1128/AEM.71.5.2771-2776.2005 |
op_rights |
Copyright © 2005, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.71.5.2771-2776.2005 |
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Applied and Environmental Microbiology |
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71 |
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5 |
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2771 |
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2776 |
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1766039684570415104 |