Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems
ABSTRACT : The Gemmatimonadota phylum has been widely detected in diverse natural environments, yet their specific ecological roles in many habitats remain poorly investigated. Similarly, the Candidatus ARS69 phylum has been identified only in a few habitats, and literature on their metabolic functi...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789658/ http://www.ncbi.nlm.nih.gov/pubmed/38229335 https://doi.org/10.1007/s00253-023-12991-6 |
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ftpubmed:oai:pubmedcentral.nih.gov:10789658 2024-02-11T10:00:51+01:00 Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems Venkatachalam, Siddarthan Jabir, Thajudeen Vipindas, Puthiya Veettil Krishnan, Kottekkatu Padinchati 2024-01-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789658/ http://www.ncbi.nlm.nih.gov/pubmed/38229335 https://doi.org/10.1007/s00253-023-12991-6 en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789658/ http://www.ncbi.nlm.nih.gov/pubmed/38229335 http://dx.doi.org/10.1007/s00253-023-12991-6 © The Author(s) 2024 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . Appl Microbiol Biotechnol Genomics Transcriptomics Proteomics Text 2024 ftpubmed https://doi.org/10.1007/s00253-023-12991-6 2024-01-21T01:47:43Z ABSTRACT : The Gemmatimonadota phylum has been widely detected in diverse natural environments, yet their specific ecological roles in many habitats remain poorly investigated. Similarly, the Candidatus ARS69 phylum has been identified only in a few habitats, and literature on their metabolic functions is relatively scarce. In the present study, we investigated the ecological significance of phyla Ca. ARS69 and Gemmatimonadota in the Arctic glacier foreland (GF) ecosystems through genome-resolved metagenomics. We have reconstructed the first high-quality metagenome-assembled genome (MAG) belonging to Ca. ARS69 and 12 other MAGs belonging to phylum Gemmatimonadota from the three different Arctic GF samples. We further elucidated these two groups phylogenetic lineage and their metabolic function through phylogenomic and pangenomic analysis. The analysis showed that all the reconstructed MAGs potentially belonged to novel species. The MAGs belonged to Ca. ARS69 consist about 8296 gene clusters, of which only about 8% of single-copy core genes (n = 980) were shared among them. The study also revealed the potential ecological role of Ca. ARS69 is associated with carbon fixation, denitrification, sulfite oxidation, and reduction biochemical processes in the GF ecosystems. Similarly, the study demonstrates the widespread distribution of different classes of Gemmatimonadota across wide ranges of ecosystems and their metabolic functions, including in the polar region. KEY POINTS: • Glacier foreland ecosystems act as a natural laboratory to study microbial community structure. • We have reconstructed 13 metagenome-assembled genomes from the soil samples. • All the reconstructed MAGs belonged to novel species with different metabolic processes. • Ca. ARS69 and Gemmatimonadota MAGs were found to participate in carbon fixation and denitrification processes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12991-6. Text Arctic PubMed Central (PMC) Arctic Applied Microbiology and Biotechnology 108 1 |
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PubMed Central (PMC) |
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ftpubmed |
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English |
topic |
Genomics Transcriptomics Proteomics |
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Genomics Transcriptomics Proteomics Venkatachalam, Siddarthan Jabir, Thajudeen Vipindas, Puthiya Veettil Krishnan, Kottekkatu Padinchati Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
topic_facet |
Genomics Transcriptomics Proteomics |
description |
ABSTRACT : The Gemmatimonadota phylum has been widely detected in diverse natural environments, yet their specific ecological roles in many habitats remain poorly investigated. Similarly, the Candidatus ARS69 phylum has been identified only in a few habitats, and literature on their metabolic functions is relatively scarce. In the present study, we investigated the ecological significance of phyla Ca. ARS69 and Gemmatimonadota in the Arctic glacier foreland (GF) ecosystems through genome-resolved metagenomics. We have reconstructed the first high-quality metagenome-assembled genome (MAG) belonging to Ca. ARS69 and 12 other MAGs belonging to phylum Gemmatimonadota from the three different Arctic GF samples. We further elucidated these two groups phylogenetic lineage and their metabolic function through phylogenomic and pangenomic analysis. The analysis showed that all the reconstructed MAGs potentially belonged to novel species. The MAGs belonged to Ca. ARS69 consist about 8296 gene clusters, of which only about 8% of single-copy core genes (n = 980) were shared among them. The study also revealed the potential ecological role of Ca. ARS69 is associated with carbon fixation, denitrification, sulfite oxidation, and reduction biochemical processes in the GF ecosystems. Similarly, the study demonstrates the widespread distribution of different classes of Gemmatimonadota across wide ranges of ecosystems and their metabolic functions, including in the polar region. KEY POINTS: • Glacier foreland ecosystems act as a natural laboratory to study microbial community structure. • We have reconstructed 13 metagenome-assembled genomes from the soil samples. • All the reconstructed MAGs belonged to novel species with different metabolic processes. • Ca. ARS69 and Gemmatimonadota MAGs were found to participate in carbon fixation and denitrification processes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12991-6. |
format |
Text |
author |
Venkatachalam, Siddarthan Jabir, Thajudeen Vipindas, Puthiya Veettil Krishnan, Kottekkatu Padinchati |
author_facet |
Venkatachalam, Siddarthan Jabir, Thajudeen Vipindas, Puthiya Veettil Krishnan, Kottekkatu Padinchati |
author_sort |
Venkatachalam, Siddarthan |
title |
Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
title_short |
Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
title_full |
Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
title_fullStr |
Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
title_full_unstemmed |
Ecological significance of Candidatus ARS69 and Gemmatimonadota in the Arctic glacier foreland ecosystems |
title_sort |
ecological significance of candidatus ars69 and gemmatimonadota in the arctic glacier foreland ecosystems |
publisher |
Springer Berlin Heidelberg |
publishDate |
2024 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789658/ http://www.ncbi.nlm.nih.gov/pubmed/38229335 https://doi.org/10.1007/s00253-023-12991-6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Appl Microbiol Biotechnol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789658/ http://www.ncbi.nlm.nih.gov/pubmed/38229335 http://dx.doi.org/10.1007/s00253-023-12991-6 |
op_rights |
© The Author(s) 2024 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1007/s00253-023-12991-6 |
container_title |
Applied Microbiology and Biotechnology |
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108 |
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1790596581014110208 |