Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon
Early microbial colonization has a profound impact on host physiology during different stages of ontogeny. Although several studies have focused on early bacterial colonization and succession, the composition and role of fungal communities are poorly known in fish. Here, we sequenced the internal tr...
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ftpubmed:oai:pubmedcentral.nih.gov:10781754 2024-02-11T10:02:04+01:00 Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon Lokesh, Jep Siriyappagouder, Prabhugouda Fernandes, Jorge M. O. 2024-01-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10781754/ http://www.ncbi.nlm.nih.gov/pubmed/38200059 https://doi.org/10.1038/s41598-023-50883-x en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10781754/ http://www.ncbi.nlm.nih.gov/pubmed/38200059 http://dx.doi.org/10.1038/s41598-023-50883-x © The Author(s) 2024 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . Sci Rep Article Text 2024 ftpubmed https://doi.org/10.1038/s41598-023-50883-x 2024-01-14T02:14:01Z Early microbial colonization has a profound impact on host physiology during different stages of ontogeny. Although several studies have focused on early bacterial colonization and succession, the composition and role of fungal communities are poorly known in fish. Here, we sequenced the internal transcribed spacer 2 (ITS2) region of fungi to profile the mycobiome associated with the eggs, hatchlings and intestine of Atlantic salmon at various freshwater and marine stages. In most of the stages studied, fungal diversity was lower than bacterial diversity. There were several stage-specific fungal phylotypes belonging to different stages of ontogeny but some groups, such as Candida tropicalis, Saccharomyces cerevisiae, Alternaria metachromatica, Davidiella tassiana and Humicola nigrescens, persisted during successive stages of ontogeny. We observed significant changes in the intestinal fungal communities during the first feeding. Prior to first feeding, Humicola nigrescens dominated, but Saccharomyces cerevisiae (10 weeks post hatch) and Candida tropicalis (12 weeks post hatch) became dominant subsequently. Seawater transfer resulted in a decrease in alpha diversity and an increase in Candida tropicalis abundance. We also observed notable variations in beta diversity and composition between the different farms. Overall, the present study sheds light on the fungal communities of Atlantic salmon from early ontogeny to adulthood. These novel findings will also be useful in future studies investigating host-microbiota interactions in the context of developing better nutritional and health management strategies for Atlantic salmon farming. Text Atlantic salmon PubMed Central (PMC) Scientific Reports 14 1 |
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Article Lokesh, Jep Siriyappagouder, Prabhugouda Fernandes, Jorge M. O. Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
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Article |
description |
Early microbial colonization has a profound impact on host physiology during different stages of ontogeny. Although several studies have focused on early bacterial colonization and succession, the composition and role of fungal communities are poorly known in fish. Here, we sequenced the internal transcribed spacer 2 (ITS2) region of fungi to profile the mycobiome associated with the eggs, hatchlings and intestine of Atlantic salmon at various freshwater and marine stages. In most of the stages studied, fungal diversity was lower than bacterial diversity. There were several stage-specific fungal phylotypes belonging to different stages of ontogeny but some groups, such as Candida tropicalis, Saccharomyces cerevisiae, Alternaria metachromatica, Davidiella tassiana and Humicola nigrescens, persisted during successive stages of ontogeny. We observed significant changes in the intestinal fungal communities during the first feeding. Prior to first feeding, Humicola nigrescens dominated, but Saccharomyces cerevisiae (10 weeks post hatch) and Candida tropicalis (12 weeks post hatch) became dominant subsequently. Seawater transfer resulted in a decrease in alpha diversity and an increase in Candida tropicalis abundance. We also observed notable variations in beta diversity and composition between the different farms. Overall, the present study sheds light on the fungal communities of Atlantic salmon from early ontogeny to adulthood. These novel findings will also be useful in future studies investigating host-microbiota interactions in the context of developing better nutritional and health management strategies for Atlantic salmon farming. |
format |
Text |
author |
Lokesh, Jep Siriyappagouder, Prabhugouda Fernandes, Jorge M. O. |
author_facet |
Lokesh, Jep Siriyappagouder, Prabhugouda Fernandes, Jorge M. O. |
author_sort |
Lokesh, Jep |
title |
Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
title_short |
Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
title_full |
Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
title_fullStr |
Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
title_full_unstemmed |
Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon |
title_sort |
unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in atlantic salmon |
publisher |
Nature Publishing Group UK |
publishDate |
2024 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10781754/ http://www.ncbi.nlm.nih.gov/pubmed/38200059 https://doi.org/10.1038/s41598-023-50883-x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10781754/ http://www.ncbi.nlm.nih.gov/pubmed/38200059 http://dx.doi.org/10.1038/s41598-023-50883-x |
op_rights |
© The Author(s) 2024 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1038/s41598-023-50883-x |
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Scientific Reports |
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