Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species
As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat toler...
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ftpubmed:oai:pubmedcentral.nih.gov:10778899 2024-02-11T10:03:14+01:00 Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species Du, Mingyang Jiang, Zhuxiang Wang, Chaogang Wei, Chenchen Li, Qingyuan Cong, Rihao Wang, Wei Zhang, Guofan Li, Li 2023-12-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10778899/ https://doi.org/10.3390/ijms25010125 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10778899/ http://dx.doi.org/10.3390/ijms25010125 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Int J Mol Sci Article Text 2023 ftpubmed https://doi.org/10.3390/ijms25010125 2024-01-14T02:06:56Z As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F(2) generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F(2) population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor (Trpm2), transcriptional factor (Gata3), protein ubiquitination (Ube2h, Usp50, Uchl3), heat shock subfamily (Dnajc17, Dnaja1), and transporters (Slc16a9, Slc16a14, Slc16a9, Slc16a2). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry. Text Crassostrea gigas PubMed Central (PMC) International Journal of Molecular Sciences 25 1 125 |
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Article Du, Mingyang Jiang, Zhuxiang Wang, Chaogang Wei, Chenchen Li, Qingyuan Cong, Rihao Wang, Wei Zhang, Guofan Li, Li Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
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As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F(2) generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F(2) population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor (Trpm2), transcriptional factor (Gata3), protein ubiquitination (Ube2h, Usp50, Uchl3), heat shock subfamily (Dnajc17, Dnaja1), and transporters (Slc16a9, Slc16a14, Slc16a9, Slc16a2). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry. |
format |
Text |
author |
Du, Mingyang Jiang, Zhuxiang Wang, Chaogang Wei, Chenchen Li, Qingyuan Cong, Rihao Wang, Wei Zhang, Guofan Li, Li |
author_facet |
Du, Mingyang Jiang, Zhuxiang Wang, Chaogang Wei, Chenchen Li, Qingyuan Cong, Rihao Wang, Wei Zhang, Guofan Li, Li |
author_sort |
Du, Mingyang |
title |
Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
title_short |
Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
title_full |
Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
title_fullStr |
Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
title_full_unstemmed |
Genome-Wide Association Analysis of Heat Tolerance in F(2) Progeny from the Hybridization between Two Congeneric Oyster Species |
title_sort |
genome-wide association analysis of heat tolerance in f(2) progeny from the hybridization between two congeneric oyster species |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10778899/ https://doi.org/10.3390/ijms25010125 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10778899/ http://dx.doi.org/10.3390/ijms25010125 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.3390/ijms25010125 |
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International Journal of Molecular Sciences |
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25 |
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1 |
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125 |
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1790599423888195584 |