Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic
Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is...
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ftpubmed:oai:pubmedcentral.nih.gov:10710508 2024-01-14T10:04:12+01:00 Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic Priest, Taylor Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. 2023-12-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710508/ http://www.ncbi.nlm.nih.gov/pubmed/38071398 https://doi.org/10.1038/s43705-023-00324-7 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710508/ http://www.ncbi.nlm.nih.gov/pubmed/38071398 http://dx.doi.org/10.1038/s43705-023-00324-7 © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . ISME Commun Article Text 2023 ftpubmed https://doi.org/10.1038/s43705-023-00324-7 2023-12-17T01:48:45Z Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is known from high-latitude waters and during later seasonal stages. Here, we combine glycan microarrays and analytical chromatography with metagenomics and metatranscriptomics to show the spatial heterogeneity in glycan distribution and potential carbohydrate utilisation by microbes in Atlantic waters of the Arctic. The composition and abundance of monomers and glycan structures in POM varied with location and depth. Complex fucose-containing sulfated polysaccharides, known to accumulate in the ocean, were consistently detected, while the more labile β-1,3-glucan exhibited a patchy distribution. Through ‘omics analysis, we identify variations in the abundance and transcription of carbohydrate degradation-related genes across samples at the community and population level. The populations contributing the most to transcription were taxonomically related to those known as primary responders and key carbohydrate degraders in temperate ecosystems, such as NS4 Marine Group and Formosa. The unique transcription profiles for these populations suggest distinct substrate utilisation potentials, with predicted glycan targets corresponding to those structurally identified in POM from the same sampling sites. By combining cutting-edge technologies and protocols, we provide insights into the carbohydrate component of the carbon cycle in the Arctic during late summer and present a high-quality dataset that will be of great value for future comparative analyses. Text Arctic Phytoplankton PubMed Central (PMC) Arctic ISME Communications 3 1 |
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Article Priest, Taylor Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
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Article |
description |
Carbohydrates are chemically and structurally diverse, represent a substantial fraction of marine organic matter and are key substrates for heterotrophic microbes. Studies on carbohydrate utilisation by marine microbes have been centred on phytoplankton blooms in temperate regions, while far less is known from high-latitude waters and during later seasonal stages. Here, we combine glycan microarrays and analytical chromatography with metagenomics and metatranscriptomics to show the spatial heterogeneity in glycan distribution and potential carbohydrate utilisation by microbes in Atlantic waters of the Arctic. The composition and abundance of monomers and glycan structures in POM varied with location and depth. Complex fucose-containing sulfated polysaccharides, known to accumulate in the ocean, were consistently detected, while the more labile β-1,3-glucan exhibited a patchy distribution. Through ‘omics analysis, we identify variations in the abundance and transcription of carbohydrate degradation-related genes across samples at the community and population level. The populations contributing the most to transcription were taxonomically related to those known as primary responders and key carbohydrate degraders in temperate ecosystems, such as NS4 Marine Group and Formosa. The unique transcription profiles for these populations suggest distinct substrate utilisation potentials, with predicted glycan targets corresponding to those structurally identified in POM from the same sampling sites. By combining cutting-edge technologies and protocols, we provide insights into the carbohydrate component of the carbon cycle in the Arctic during late summer and present a high-quality dataset that will be of great value for future comparative analyses. |
format |
Text |
author |
Priest, Taylor Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. |
author_facet |
Priest, Taylor Vidal-Melgosa, Silvia Hehemann, Jan-Hendrik Amann, Rudolf Fuchs, Bernhard M. |
author_sort |
Priest, Taylor |
title |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_short |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_full |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_fullStr |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_full_unstemmed |
Carbohydrates and carbohydrate degradation gene abundance and transcription in Atlantic waters of the Arctic |
title_sort |
carbohydrates and carbohydrate degradation gene abundance and transcription in atlantic waters of the arctic |
publisher |
Nature Publishing Group UK |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710508/ http://www.ncbi.nlm.nih.gov/pubmed/38071398 https://doi.org/10.1038/s43705-023-00324-7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Phytoplankton |
genre_facet |
Arctic Phytoplankton |
op_source |
ISME Commun |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710508/ http://www.ncbi.nlm.nih.gov/pubmed/38071398 http://dx.doi.org/10.1038/s43705-023-00324-7 |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1038/s43705-023-00324-7 |
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ISME Communications |
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3 |
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