Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease
In the Chilean salmon farming industry, infection by Piscirickettsia salmonis is the primary cause of the main bacterial disease known as Piscirickettsiosis, which has an overwhelming economic impact. Although it has been demonstrated that Piscirickettsiosis modifies the expression of numerous salmo...
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ftpubmed:oai:pubmedcentral.nih.gov:10696053 2024-01-07T09:42:13+01:00 Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease Bravo, Scarleth Moya, Javier Leiva, Francisco Guzman, Osiel Vidal, Rodrigo 2023-11-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696053/ http://www.ncbi.nlm.nih.gov/pubmed/38058636 https://doi.org/10.1016/j.heliyon.2023.e22377 en eng Elsevier http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696053/ http://www.ncbi.nlm.nih.gov/pubmed/38058636 http://dx.doi.org/10.1016/j.heliyon.2023.e22377 © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Heliyon Research Article Text 2023 ftpubmed https://doi.org/10.1016/j.heliyon.2023.e22377 2023-12-10T01:50:07Z In the Chilean salmon farming industry, infection by Piscirickettsia salmonis is the primary cause of the main bacterial disease known as Piscirickettsiosis, which has an overwhelming economic impact. Although it has been demonstrated that Piscirickettsiosis modifies the expression of numerous salmonids genes, it is yet unknown how alternative splicing (AS) contributes to salmonids bacterial infection. AS, has the potential to create heterogeneity at the protein and RNA levels and has been associated as a relevant molecular mechanism in the immune response of eukaryotes to several diseases. In this study, we used RNA data to survey P. salmonis-induced modifications in the AS of Atlantic salmon and found that P. salmonis infection promoted a substantial number (158,668) of AS events. Differentially spliced genes (DSG) sensitive to Piscirickettsiosis were predominantly enriched in genes involved in RNA processing, splicing and spliceosome processes (e.g., hnRNPm, hnRPc, SRSF7, SRSF45), whereas among the DSG of resistant and susceptible to Piscirickettsiosis, several metabolic and immune processes were found, most notably associated to the regulation of GTPase, lysosome and telomere organization-maintenance. Furthermore, we found that DSG were mostly not differentially expressed (5–7 %) and were implicated in distinct biological pathways. Therefore, our results underpin AS achieving a significant regulatory performance in the response of salmonids to Piscirickettsiosis. Text Atlantic salmon PubMed Central (PMC) Heliyon 9 11 e22377 |
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Research Article Bravo, Scarleth Moya, Javier Leiva, Francisco Guzman, Osiel Vidal, Rodrigo Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
topic_facet |
Research Article |
description |
In the Chilean salmon farming industry, infection by Piscirickettsia salmonis is the primary cause of the main bacterial disease known as Piscirickettsiosis, which has an overwhelming economic impact. Although it has been demonstrated that Piscirickettsiosis modifies the expression of numerous salmonids genes, it is yet unknown how alternative splicing (AS) contributes to salmonids bacterial infection. AS, has the potential to create heterogeneity at the protein and RNA levels and has been associated as a relevant molecular mechanism in the immune response of eukaryotes to several diseases. In this study, we used RNA data to survey P. salmonis-induced modifications in the AS of Atlantic salmon and found that P. salmonis infection promoted a substantial number (158,668) of AS events. Differentially spliced genes (DSG) sensitive to Piscirickettsiosis were predominantly enriched in genes involved in RNA processing, splicing and spliceosome processes (e.g., hnRNPm, hnRPc, SRSF7, SRSF45), whereas among the DSG of resistant and susceptible to Piscirickettsiosis, several metabolic and immune processes were found, most notably associated to the regulation of GTPase, lysosome and telomere organization-maintenance. Furthermore, we found that DSG were mostly not differentially expressed (5–7 %) and were implicated in distinct biological pathways. Therefore, our results underpin AS achieving a significant regulatory performance in the response of salmonids to Piscirickettsiosis. |
format |
Text |
author |
Bravo, Scarleth Moya, Javier Leiva, Francisco Guzman, Osiel Vidal, Rodrigo |
author_facet |
Bravo, Scarleth Moya, Javier Leiva, Francisco Guzman, Osiel Vidal, Rodrigo |
author_sort |
Bravo, Scarleth |
title |
Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
title_short |
Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
title_full |
Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
title_fullStr |
Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
title_full_unstemmed |
Transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of Piscirickettsiosis disease |
title_sort |
transcriptome analyses reveal key roles of alternative splicing regulation in atlantic salmon during the infectious process of piscirickettsiosis disease |
publisher |
Elsevier |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696053/ http://www.ncbi.nlm.nih.gov/pubmed/38058636 https://doi.org/10.1016/j.heliyon.2023.e22377 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Heliyon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696053/ http://www.ncbi.nlm.nih.gov/pubmed/38058636 http://dx.doi.org/10.1016/j.heliyon.2023.e22377 |
op_rights |
© 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.1016/j.heliyon.2023.e22377 |
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Heliyon |
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9 |
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11 |
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e22377 |
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1787423156836761600 |