The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater
There is a renewed interest in hydrocarbon biodegradation in Arctic seawaters due to increasing ship traffic and risk for oil spills. Most studies, however, fail to address the effect of increasing pressure as an environmental parameter. Here, we conducted a series of pressurized enrichments (0.1–30...
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ftpubmed:oai:pubmedcentral.nih.gov:10686064 2023-12-31T10:02:46+01:00 The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater Marietou, Angeliki Schmidt, Jennie Spicker Rasmussen, Martin R. Scoma, Alberto Rysgaard, Søren Vergeynst, Leendert 2023-11-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686064/ http://www.ncbi.nlm.nih.gov/pubmed/37943057 https://doi.org/10.1128/aem.00987-23 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686064/ http://www.ncbi.nlm.nih.gov/pubmed/37943057 http://dx.doi.org/10.1128/aem.00987-23 Copyright © 2023 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2 All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . Appl Environ Microbiol Biodegradation Text 2023 ftpubmed https://doi.org/10.1128/aem.00987-23 2023-12-03T02:06:37Z There is a renewed interest in hydrocarbon biodegradation in Arctic seawaters due to increasing ship traffic and risk for oil spills. Most studies, however, fail to address the effect of increasing pressure as an environmental parameter. Here, we conducted a series of pressurized enrichments (0.1–30 MPa, 4°C) inoculated with a 100-day-old hydrocarbon-degrading biofilm collected from 615 m deep in Arctic seawater. Cell-specific CO(2) production rates provided a clear summary of the observed microbial activity: a bloom of a hydrocarbon degrading-biofilm generating 0.82–0.90 fmol CO(2)⋅bacterial gene(−1)⋅day(−1) at 0.1–8 MPa, but undetectable activity at 30 MPa until day 6. At 30 MPa, the microbial activity increased between days 6 and 34 with an average rate of 0.36 ± 0.08 fmol CO(2)⋅bacterial gene(−1)⋅day(−1). Amplicon sequencing revealed no differences in the microbial community composition at 0.1–12 MPa. While the typical Arctic alkane degraders Oleispira and Shewanella were abundant across all hydrostatic pressures and over time, Colwellia, Neptunomonas, and Kiloniella were significantly enriched solely at 30 MPa. Our results suggest that the physiological adaptations of psychrophilic bacteria to thrive at sub-zero temperature make Arctic oil degraders tolerant to mild hydrostatic pressures of up to 12 MPa, whereas temperate climate communities have shown hydrostatic pressure-induced inhibition at 10–15 MPa in comparable studies. The activity of hydrocarbon degraders in sinking marine oil snow in the Arctic may remain unaffected down to depths of about 1,200 m, after which hydrostatic pressure can significantly affect hydrocarbon degradation at increasing depths down to 3,000 m. IMPORTANCE: Increased ship traffic in the Arctic region raises the risk of oil spills. With an average sea depth of 1,000 m, there is a growing concern over the potential release of oil sinking in the form of marine oil snow into deep Arctic waters. At increasing depth, the oil-degrading community is exposed to increasing hydrostatic ... Text Arctic PubMed Central (PMC) Applied and Environmental Microbiology 89 11 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
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Biodegradation |
spellingShingle |
Biodegradation Marietou, Angeliki Schmidt, Jennie Spicker Rasmussen, Martin R. Scoma, Alberto Rysgaard, Søren Vergeynst, Leendert The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
topic_facet |
Biodegradation |
description |
There is a renewed interest in hydrocarbon biodegradation in Arctic seawaters due to increasing ship traffic and risk for oil spills. Most studies, however, fail to address the effect of increasing pressure as an environmental parameter. Here, we conducted a series of pressurized enrichments (0.1–30 MPa, 4°C) inoculated with a 100-day-old hydrocarbon-degrading biofilm collected from 615 m deep in Arctic seawater. Cell-specific CO(2) production rates provided a clear summary of the observed microbial activity: a bloom of a hydrocarbon degrading-biofilm generating 0.82–0.90 fmol CO(2)⋅bacterial gene(−1)⋅day(−1) at 0.1–8 MPa, but undetectable activity at 30 MPa until day 6. At 30 MPa, the microbial activity increased between days 6 and 34 with an average rate of 0.36 ± 0.08 fmol CO(2)⋅bacterial gene(−1)⋅day(−1). Amplicon sequencing revealed no differences in the microbial community composition at 0.1–12 MPa. While the typical Arctic alkane degraders Oleispira and Shewanella were abundant across all hydrostatic pressures and over time, Colwellia, Neptunomonas, and Kiloniella were significantly enriched solely at 30 MPa. Our results suggest that the physiological adaptations of psychrophilic bacteria to thrive at sub-zero temperature make Arctic oil degraders tolerant to mild hydrostatic pressures of up to 12 MPa, whereas temperate climate communities have shown hydrostatic pressure-induced inhibition at 10–15 MPa in comparable studies. The activity of hydrocarbon degraders in sinking marine oil snow in the Arctic may remain unaffected down to depths of about 1,200 m, after which hydrostatic pressure can significantly affect hydrocarbon degradation at increasing depths down to 3,000 m. IMPORTANCE: Increased ship traffic in the Arctic region raises the risk of oil spills. With an average sea depth of 1,000 m, there is a growing concern over the potential release of oil sinking in the form of marine oil snow into deep Arctic waters. At increasing depth, the oil-degrading community is exposed to increasing hydrostatic ... |
format |
Text |
author |
Marietou, Angeliki Schmidt, Jennie Spicker Rasmussen, Martin R. Scoma, Alberto Rysgaard, Søren Vergeynst, Leendert |
author_facet |
Marietou, Angeliki Schmidt, Jennie Spicker Rasmussen, Martin R. Scoma, Alberto Rysgaard, Søren Vergeynst, Leendert |
author_sort |
Marietou, Angeliki |
title |
The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
title_short |
The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
title_full |
The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
title_fullStr |
The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
title_full_unstemmed |
The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater |
title_sort |
effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in arctic seawater |
publisher |
American Society for Microbiology |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686064/ http://www.ncbi.nlm.nih.gov/pubmed/37943057 https://doi.org/10.1128/aem.00987-23 |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Appl Environ Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686064/ http://www.ncbi.nlm.nih.gov/pubmed/37943057 http://dx.doi.org/10.1128/aem.00987-23 |
op_rights |
Copyright © 2023 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2 All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . |
op_doi |
https://doi.org/10.1128/aem.00987-23 |
container_title |
Applied and Environmental Microbiology |
container_volume |
89 |
container_issue |
11 |
_version_ |
1786814355729285120 |