Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill

Antarctic krill (Euphausia superba) is a key species of the Antarctic ecosystem whose unique ecological status and great development potential have attracted extensive attention. However, the genomic characteristics and potential biological functions of the symbiotic microorganisms of Antarctic kril...

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Bibliographic Details
Published in:Microorganisms
Main Authors: Feng, Zhengqi, Wang, Yuanyuan, Ma, Lingbo, Huang, Shanzi, Wang, Lumin, He, Jianguo, Guo, Changjun
Format: Text
Language:English
Published: MDPI 2023
Subjects:
Article
Antarc*
Antarctic
Antarctic Krill
Euphausia superba
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10536100/
http://www.ncbi.nlm.nih.gov/pubmed/37764125
https://doi.org/10.3390/microorganisms11092281
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Summary:Antarctic krill (Euphausia superba) is a key species of the Antarctic ecosystem whose unique ecological status and great development potential have attracted extensive attention. However, the genomic characteristics and potential biological functions of the symbiotic microorganisms of Antarctic krill remain unknown. In this study, we cultured and identified a strain of Brucella sp. WY7 from Antarctic krill using whole-genome sequencing and assembly, functional annotation, and comparative genomics analysis. First, based on 16S rDNA sequence alignment and phylogenetic tree analysis, we identified strain WY7 as Brucella. The assembled genome of strain WY7 revealed that it has two chromosomes and a plasmid, with a total genome length of 4,698,850 bp and an average G + C content of 57.18%. The DNA—DNA hybridization value and average nucleotide identity value of strain WY7 and Brucella anthropi ATCC(®) 49188(TM), a type strain isolated from human clinical specimens, were 94.8% and 99.07%, respectively, indicating that strain WY7 is closely related to Brucella anthropi. Genomic island prediction showed that the strain has 60 genomic islands, which may produce HigB and VapC toxins. AntiSMASH analysis results showed that strain WY7 might produce many secondary metabolites, such as terpenes, siderophores and ectoine. Moreover, the genome contains genes involved in the degradation of aromatic compounds, suggesting that strain WY7 can use aromatic compounds in its metabolism. Our work will help to understand the genomic characteristics and metabolic potential of bacterial strains isolated from Antarctic krill, thereby revealing their roles in Antarctic krill and marine ecosystems.

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