Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp.
Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, their large-scale production and application are restricted by high production costs. Such costs can be reduced using renewable raw materials...
Published in: | Applied Biochemistry and Biotechnology |
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511570/ http://www.ncbi.nlm.nih.gov/pubmed/36811772 https://doi.org/10.1007/s12010-023-04317-z |
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ftpubmed:oai:pubmedcentral.nih.gov:10511570 2023-10-09T21:45:45+02:00 Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. Faria, Nuno Torres Nascimento, Miguel Figueiredo Ferreira, Flávio Alves Esteves, Teresa Santos, Marisa Viegas Ferreira, Frederico Castelo 2023-02-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511570/ http://www.ncbi.nlm.nih.gov/pubmed/36811772 https://doi.org/10.1007/s12010-023-04317-z en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511570/ http://www.ncbi.nlm.nih.gov/pubmed/36811772 http://dx.doi.org/10.1007/s12010-023-04317-z © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . Appl Biochem Biotechnol Original Article Text 2023 ftpubmed https://doi.org/10.1007/s12010-023-04317-z 2023-09-24T00:59:56Z Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, their large-scale production and application are restricted by high production costs. Such costs can be reduced using renewable raw materials and facilitated downstream processing. Here, a novel strategy for mannosylerythritol lipid (MEL) production explores the combination of hydrophilic and hydrophobic carbon sources sideways with a novel downstream processing strategy, based on nanofiltration technology. Co-substrate MEL production by Moesziomyces antarcticus was threefold higher than using D-glucose with low levels of residual lipids. The use of waste frying oil instead of soybean oil (SBO) in co-substrate strategy resulted in similar MEL production. Moesziomyces antarcticus cultivations, using 3.9 M of total carbon in substrates, yields 7.3, 18.1, and 20.1 g/L of MEL, and 2.1, 10.0, and 5.1 g/L of residual lipids, for D-glucose, SBO, and a combination of D-Glucose and SBO, respectively. Such approach makes it possible to reduce the amount of oil used, offset by the equivalent molar increase in D-glucose, improving sustainability and decreasing residual unconsumed oil substrates, facilitating downstream processing. Moesziomyces spp. also produces lipases that broken down the oil and, thus, residual unconsumed oils are in the form of free fatty-acids or monoacylglycerol, which are smaller molecules than MEL. Therefore, nanofiltration of ethyl acetate extracts from co-substrate-based culture broths allows to improve MEL purity (ratio of MEL per total MEL and residual lipids) from 66 to 93% using 3-diavolumes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12010-023-04317-z. Text Antarc* antarcticus PubMed Central (PMC) Applied Biochemistry and Biotechnology 195 10 6132 6149 |
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Original Article |
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Original Article Faria, Nuno Torres Nascimento, Miguel Figueiredo Ferreira, Flávio Alves Esteves, Teresa Santos, Marisa Viegas Ferreira, Frederico Castelo Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
topic_facet |
Original Article |
description |
Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, their large-scale production and application are restricted by high production costs. Such costs can be reduced using renewable raw materials and facilitated downstream processing. Here, a novel strategy for mannosylerythritol lipid (MEL) production explores the combination of hydrophilic and hydrophobic carbon sources sideways with a novel downstream processing strategy, based on nanofiltration technology. Co-substrate MEL production by Moesziomyces antarcticus was threefold higher than using D-glucose with low levels of residual lipids. The use of waste frying oil instead of soybean oil (SBO) in co-substrate strategy resulted in similar MEL production. Moesziomyces antarcticus cultivations, using 3.9 M of total carbon in substrates, yields 7.3, 18.1, and 20.1 g/L of MEL, and 2.1, 10.0, and 5.1 g/L of residual lipids, for D-glucose, SBO, and a combination of D-Glucose and SBO, respectively. Such approach makes it possible to reduce the amount of oil used, offset by the equivalent molar increase in D-glucose, improving sustainability and decreasing residual unconsumed oil substrates, facilitating downstream processing. Moesziomyces spp. also produces lipases that broken down the oil and, thus, residual unconsumed oils are in the form of free fatty-acids or monoacylglycerol, which are smaller molecules than MEL. Therefore, nanofiltration of ethyl acetate extracts from co-substrate-based culture broths allows to improve MEL purity (ratio of MEL per total MEL and residual lipids) from 66 to 93% using 3-diavolumes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12010-023-04317-z. |
format |
Text |
author |
Faria, Nuno Torres Nascimento, Miguel Figueiredo Ferreira, Flávio Alves Esteves, Teresa Santos, Marisa Viegas Ferreira, Frederico Castelo |
author_facet |
Faria, Nuno Torres Nascimento, Miguel Figueiredo Ferreira, Flávio Alves Esteves, Teresa Santos, Marisa Viegas Ferreira, Frederico Castelo |
author_sort |
Faria, Nuno Torres |
title |
Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
title_short |
Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
title_full |
Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
title_fullStr |
Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
title_full_unstemmed |
Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp. |
title_sort |
substrates of opposite polarities and downstream processing for efficient production of the biosurfactant mannosylerythritol lipids from moesziomyces spp. |
publisher |
Springer US |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511570/ http://www.ncbi.nlm.nih.gov/pubmed/36811772 https://doi.org/10.1007/s12010-023-04317-z |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Appl Biochem Biotechnol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511570/ http://www.ncbi.nlm.nih.gov/pubmed/36811772 http://dx.doi.org/10.1007/s12010-023-04317-z |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1007/s12010-023-04317-z |
container_title |
Applied Biochemistry and Biotechnology |
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195 |
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10 |
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6132 |
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6149 |
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1779321403728723968 |