Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas
Coastal environments commonly experience fluctuations in salinity and hypoxia–reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and funct...
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ftpubmed:oai:pubmedcentral.nih.gov:10445735 2023-09-26T15:17:24+02:00 Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas Steffen, Jennifer B. M. Sokolov, Eugene P. Bock, Christian Sokolova, Inna M. 2023-08-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445735/ http://www.ncbi.nlm.nih.gov/pubmed/37470191 https://doi.org/10.1242/jeb.246164 en eng The Company of Biologists Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445735/ http://www.ncbi.nlm.nih.gov/pubmed/37470191 http://dx.doi.org/10.1242/jeb.246164 © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. J Exp Biol Research Article Text 2023 ftpubmed https://doi.org/10.1242/jeb.246164 2023-08-27T01:12:20Z Coastal environments commonly experience fluctuations in salinity and hypoxia–reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood. We determined the rates of respiration and reactive oxygen species (ROS) efflux in the mitochondria of oysters, Crassostrea gigas, acclimated to high (33 psu) or low (15 psu) salinity, and exposed to either normoxic conditions (control; 21% O(2)) or short-term hypoxia (24 h at <0.01% O(2)) and subsequent reoxygenation (1.5 h at 21% O(2)). Further, we exposed isolated mitochondria to anoxia in vitro to assess their ability to recover from acute (∼10 min) oxygen deficiency (<0.01% O(2)). Our results showed that mitochondria of oysters acclimated to high or low salinity did not show severe damage and dysfunction during H/R stress, consistent with the hypoxia tolerance of C. gigas. However, acclimation to low salinity led to improved mitochondrial performance and plasticity, indicating that 15 psu might be closer to the metabolic optimum of C. gigas than 33 psu. Thus, acclimation to low salinity increased mitochondrial oxidative phosphorylation rate and coupling efficiency and stimulated mitochondrial respiration after acute H/R stress. However, elevated ROS efflux in the mitochondria of low-salinity-acclimated oysters after acute H/R stress indicates a possible trade-off of higher respiration. The high plasticity and stress tolerance of C. gigas mitochondria may contribute to the success of this invasive species and facilitate its further expansion into brackish regions such as the Baltic Sea. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Journal of Experimental Biology 226 15 |
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Research Article Steffen, Jennifer B. M. Sokolov, Eugene P. Bock, Christian Sokolova, Inna M. Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
topic_facet |
Research Article |
description |
Coastal environments commonly experience fluctuations in salinity and hypoxia–reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood. We determined the rates of respiration and reactive oxygen species (ROS) efflux in the mitochondria of oysters, Crassostrea gigas, acclimated to high (33 psu) or low (15 psu) salinity, and exposed to either normoxic conditions (control; 21% O(2)) or short-term hypoxia (24 h at <0.01% O(2)) and subsequent reoxygenation (1.5 h at 21% O(2)). Further, we exposed isolated mitochondria to anoxia in vitro to assess their ability to recover from acute (∼10 min) oxygen deficiency (<0.01% O(2)). Our results showed that mitochondria of oysters acclimated to high or low salinity did not show severe damage and dysfunction during H/R stress, consistent with the hypoxia tolerance of C. gigas. However, acclimation to low salinity led to improved mitochondrial performance and plasticity, indicating that 15 psu might be closer to the metabolic optimum of C. gigas than 33 psu. Thus, acclimation to low salinity increased mitochondrial oxidative phosphorylation rate and coupling efficiency and stimulated mitochondrial respiration after acute H/R stress. However, elevated ROS efflux in the mitochondria of low-salinity-acclimated oysters after acute H/R stress indicates a possible trade-off of higher respiration. The high plasticity and stress tolerance of C. gigas mitochondria may contribute to the success of this invasive species and facilitate its further expansion into brackish regions such as the Baltic Sea. |
format |
Text |
author |
Steffen, Jennifer B. M. Sokolov, Eugene P. Bock, Christian Sokolova, Inna M. |
author_facet |
Steffen, Jennifer B. M. Sokolov, Eugene P. Bock, Christian Sokolova, Inna M. |
author_sort |
Steffen, Jennifer B. M. |
title |
Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
title_short |
Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
title_full |
Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas |
title_sort |
combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the pacific oyster, crassostrea gigas |
publisher |
The Company of Biologists Ltd |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445735/ http://www.ncbi.nlm.nih.gov/pubmed/37470191 https://doi.org/10.1242/jeb.246164 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
J Exp Biol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445735/ http://www.ncbi.nlm.nih.gov/pubmed/37470191 http://dx.doi.org/10.1242/jeb.246164 |
op_rights |
© 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
op_doi |
https://doi.org/10.1242/jeb.246164 |
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Journal of Experimental Biology |
container_volume |
226 |
container_issue |
15 |
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1778138868839612416 |